A single coxsackievirus B2 capsid residue controls cytolysis and apoptosis in rhabdomyosarcoma cells

Maria Gullberg, Conny Tolf, Nina Jonsson, Charlotta Polacek, Jana Precechtelova, Miriam Badurova, Martin Sojka, Camilla Mohlin, Stina Israelsson, Kjell Johansson, Shubhada Bopegamage, Susan Hafenstein, A. Michael Lindberg

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Coxsackievirus B2 (CVB2), one of six human pathogens of the group B coxsackieviruses within the enterovirus genus of Picornaviridae, causes a wide spectrum of human diseases ranging from mild upper respiratory illnesses to myocarditis and meningitis. The CVB2 prototype strain Ohio-1 (CVB2O) was originally isolated from a patient with summer grippe in the 1950s. Later on, CVB2O was adapted to cytolytic replication in rhabdomyosarcoma (RD) cells. Here, we present analyses of the correlation between the adaptive mutations of this RD variant and the cytolytic infection in RD cells. Using reverse genetics, we identified a single amino acid change within the exposed region of the VP1 protein (glutamine to lysine at position 164) as the determinant for the acquired cytolytic trait. Moreover, this cytolytic virus induced apoptosis, including caspase activation and DNA degradation, in RD cells. These findings contribute to our understanding of the host cell adaptation process of CVB2O and provide a valuable tool for further studies of virus-host interactions.

Original languageEnglish (US)
Pages (from-to)5868-5879
Number of pages12
JournalJournal of virology
Volume84
Issue number12
DOIs
StatePublished - Jun 2010

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Gullberg, M., Tolf, C., Jonsson, N., Polacek, C., Precechtelova, J., Badurova, M., Sojka, M., Mohlin, C., Israelsson, S., Johansson, K., Bopegamage, S., Hafenstein, S., & Lindberg, A. M. (2010). A single coxsackievirus B2 capsid residue controls cytolysis and apoptosis in rhabdomyosarcoma cells. Journal of virology, 84(12), 5868-5879. https://doi.org/10.1128/JVI.02383-09