A single-nucleotide polymorphism in a Plasmodium berghei ApiAP2 transcription factor alters the development of host immunity

Munir Akkaya, Abhisheka Bansal, Patrick W. Sheehan, Mirna Pena, Alvaro Molina-Cruz, Lindsey M. Orchard, Clare K. Cimperman, Chen Feng Qi, Philipp Ross, Takele Yazew, Daniel Sturdevant, Sarah L. Anzick, Girija Thiruvengadam, Thomas Dan Otto, Oliver Billker, Manuel Llinás, Louis H. Miller, Susan K. Pierce

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The acquisition of malaria immunity is both remarkably slow and unpredictable. At present, we know little about the malaria parasite genes that influence the host’s ability to mount a protective immune response. Here, we show that a single-nucleotide polymorphism (SNP) resulting in a single amino acid change (S to F) in an ApiAP2 transcription factor in the rodent malaria parasite Plasmodium berghei (Pb) NK65 allowed infected mice to mount a T helper cell 1 (TH1)–type immune response that controlled subsequent infections. As compared to PbNK65S, PbNK65F parasites differentially expressed 46 genes, most of which are predicted to play roles in immune evasion. PbNK65F infections resulted in an early interferon-γ response and a later expansion of germinal centers, resulting in high levels of infected red blood cell–specific TH1-type immunoglobulin G2b (IgG2b) and IgG2c antibodies. Thus, the Pb ApiAP2 transcription factor functions as a critical parasite virulence factor in malaria infections.

Original languageEnglish (US)
Article numbereaaw6957
JournalScience Advances
Volume6
Issue number6
DOIs
StatePublished - Feb 5 2020

All Science Journal Classification (ASJC) codes

  • General

Fingerprint Dive into the research topics of 'A single-nucleotide polymorphism in a Plasmodium berghei ApiAP2 transcription factor alters the development of host immunity'. Together they form a unique fingerprint.

Cite this