A single-shot approach for measuring two-photon action cross-section of fluorescent markers

Kebin Shi, Ahmed A. Heikal, Zhiwen Liu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We report on a novel method designed for measuring two-photon action cross sections spectra in a single shot without tuning the excitation wavelength. Our technique is based on (i) using a nonlinear photonic crystal fiber to broaden the spectrum of the femtosecond excitation pulses and (ii) exploiting angular dispersion to focus different wavelengths to different lateral positions. As a result, two-photon fluorescence signal at different excitation wavelengths can be obtained simultaneously. As a proof of principle, the relative two-photon action cross sections of rhodamine green and DiI-C18 are measured over 740 - 860 nm range using fluorescein as a reference. Our results are in good agreement with that obtained using conventional tunable mode-locked laser.

Original languageEnglish (US)
Pages (from-to)8722-8727
Number of pages6
JournalOptics Express
Volume14
Issue number19
DOIs
StatePublished - Jan 1 2006

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markers
shot
cross sections
photons
wavelengths
excitation
rhodamine
tuning
photonics
fluorescence
fibers
pulses
crystals
lasers

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

Cite this

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title = "A single-shot approach for measuring two-photon action cross-section of fluorescent markers",
abstract = "We report on a novel method designed for measuring two-photon action cross sections spectra in a single shot without tuning the excitation wavelength. Our technique is based on (i) using a nonlinear photonic crystal fiber to broaden the spectrum of the femtosecond excitation pulses and (ii) exploiting angular dispersion to focus different wavelengths to different lateral positions. As a result, two-photon fluorescence signal at different excitation wavelengths can be obtained simultaneously. As a proof of principle, the relative two-photon action cross sections of rhodamine green and DiI-C18 are measured over 740 - 860 nm range using fluorescein as a reference. Our results are in good agreement with that obtained using conventional tunable mode-locked laser.",
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A single-shot approach for measuring two-photon action cross-section of fluorescent markers. / Shi, Kebin; Heikal, Ahmed A.; Liu, Zhiwen.

In: Optics Express, Vol. 14, No. 19, 01.01.2006, p. 8722-8727.

Research output: Contribution to journalArticle

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