TY - JOUR
T1 - A smartphone-based chloridometer for point-of-care diagnostics of cystic fibrosis
AU - Zhang, Chenji
AU - Kim, Jimin P.
AU - Creer, Michael
AU - Yang, Jian
AU - Liu, Zhiwen
N1 - Funding Information:
This work was supported in part by National Institutes of Health (NIH – United States) Awards (NIBIB EB012575, NCI CA182670) and by Vodafone Americas Foundation.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/11/15
Y1 - 2017/11/15
N2 - Chloride in sweat is an important diagnostic marker for cystic fibrosis (CF), but the implementation of point-of-care systems for diagnosis is hindered by the prohibitive costs of existing chloride sensors. To enable low cost diagnostic solutions, we recently established a citrate-derived synthesis platform for the development of new fluorescence sensors with high selectivity for chloride. As a next step, we herein designed a smartphone operated chloridometer that optimizes the analytical performance of the citrate-derived sensor materials for the detection of chloride in sweat. The sensor material demonstrated a wide linear range of 0.8–200 mM chloride and a diffusion-limited response time; sweat chloride levels corresponded to measurable changes in fluorescence emission that was captured by a smartphone. Clinical validation was performed with sweat from individuals with and without CF, demonstrating convenient sweat diagnostics with reliable detection of cystic fibrosis. To our knowledge, this is the first clinical study of a smartphone-based chloride sensor, paving the way for point-of-care diagnostic systems for CF.
AB - Chloride in sweat is an important diagnostic marker for cystic fibrosis (CF), but the implementation of point-of-care systems for diagnosis is hindered by the prohibitive costs of existing chloride sensors. To enable low cost diagnostic solutions, we recently established a citrate-derived synthesis platform for the development of new fluorescence sensors with high selectivity for chloride. As a next step, we herein designed a smartphone operated chloridometer that optimizes the analytical performance of the citrate-derived sensor materials for the detection of chloride in sweat. The sensor material demonstrated a wide linear range of 0.8–200 mM chloride and a diffusion-limited response time; sweat chloride levels corresponded to measurable changes in fluorescence emission that was captured by a smartphone. Clinical validation was performed with sweat from individuals with and without CF, demonstrating convenient sweat diagnostics with reliable detection of cystic fibrosis. To our knowledge, this is the first clinical study of a smartphone-based chloride sensor, paving the way for point-of-care diagnostic systems for CF.
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U2 - 10.1016/j.bios.2017.05.048
DO - 10.1016/j.bios.2017.05.048
M3 - Article
C2 - 28595077
AN - SCOPUS:85020314566
SN - 0956-5663
VL - 97
SP - 164
EP - 168
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -