Abstract

Chloride in sweat is an important diagnostic marker for cystic fibrosis (CF), but the implementation of point-of-care systems for diagnosis is hindered by the prohibitive costs of existing chloride sensors. To enable low cost diagnostic solutions, we recently established a citrate-derived synthesis platform for the development of new fluorescence sensors with high selectivity for chloride. As a next step, we herein designed a smartphone operated chloridometer that optimizes the analytical performance of the citrate-derived sensor materials for the detection of chloride in sweat. The sensor material demonstrated a wide linear range of 0.8–200 mM chloride and a diffusion-limited response time; sweat chloride levels corresponded to measurable changes in fluorescence emission that was captured by a smartphone. Clinical validation was performed with sweat from individuals with and without CF, demonstrating convenient sweat diagnostics with reliable detection of cystic fibrosis. To our knowledge, this is the first clinical study of a smartphone-based chloride sensor, paving the way for point-of-care diagnostic systems for CF.

Original languageEnglish (US)
Pages (from-to)164-168
Number of pages5
JournalBiosensors and Bioelectronics
Volume97
DOIs
StatePublished - Nov 15 2017

Fingerprint

Point-of-Care Systems
Smartphones
Cystic Fibrosis
Chlorides
Sweat
Sensors
Fluorescence
Citric Acid
Costs and Cost Analysis
Costs
Smartphone
Reaction Time

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

@article{0b822a87a32b4b18a3ed8920b4e9e95c,
title = "A smartphone-based chloridometer for point-of-care diagnostics of cystic fibrosis",
abstract = "Chloride in sweat is an important diagnostic marker for cystic fibrosis (CF), but the implementation of point-of-care systems for diagnosis is hindered by the prohibitive costs of existing chloride sensors. To enable low cost diagnostic solutions, we recently established a citrate-derived synthesis platform for the development of new fluorescence sensors with high selectivity for chloride. As a next step, we herein designed a smartphone operated chloridometer that optimizes the analytical performance of the citrate-derived sensor materials for the detection of chloride in sweat. The sensor material demonstrated a wide linear range of 0.8–200 mM chloride and a diffusion-limited response time; sweat chloride levels corresponded to measurable changes in fluorescence emission that was captured by a smartphone. Clinical validation was performed with sweat from individuals with and without CF, demonstrating convenient sweat diagnostics with reliable detection of cystic fibrosis. To our knowledge, this is the first clinical study of a smartphone-based chloride sensor, paving the way for point-of-care diagnostic systems for CF.",
author = "Chenji Zhang and Kim, {Jimin P.} and Michael Creer and Jian Yang and Zhiwen Liu",
year = "2017",
month = "11",
day = "15",
doi = "10.1016/j.bios.2017.05.048",
language = "English (US)",
volume = "97",
pages = "164--168",
journal = "Biosensors and Bioelectronics",
issn = "0956-5663",
publisher = "Elsevier Ltd",

}

A smartphone-based chloridometer for point-of-care diagnostics of cystic fibrosis. / Zhang, Chenji; Kim, Jimin P.; Creer, Michael; Yang, Jian; Liu, Zhiwen.

In: Biosensors and Bioelectronics, Vol. 97, 15.11.2017, p. 164-168.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A smartphone-based chloridometer for point-of-care diagnostics of cystic fibrosis

AU - Zhang, Chenji

AU - Kim, Jimin P.

AU - Creer, Michael

AU - Yang, Jian

AU - Liu, Zhiwen

PY - 2017/11/15

Y1 - 2017/11/15

N2 - Chloride in sweat is an important diagnostic marker for cystic fibrosis (CF), but the implementation of point-of-care systems for diagnosis is hindered by the prohibitive costs of existing chloride sensors. To enable low cost diagnostic solutions, we recently established a citrate-derived synthesis platform for the development of new fluorescence sensors with high selectivity for chloride. As a next step, we herein designed a smartphone operated chloridometer that optimizes the analytical performance of the citrate-derived sensor materials for the detection of chloride in sweat. The sensor material demonstrated a wide linear range of 0.8–200 mM chloride and a diffusion-limited response time; sweat chloride levels corresponded to measurable changes in fluorescence emission that was captured by a smartphone. Clinical validation was performed with sweat from individuals with and without CF, demonstrating convenient sweat diagnostics with reliable detection of cystic fibrosis. To our knowledge, this is the first clinical study of a smartphone-based chloride sensor, paving the way for point-of-care diagnostic systems for CF.

AB - Chloride in sweat is an important diagnostic marker for cystic fibrosis (CF), but the implementation of point-of-care systems for diagnosis is hindered by the prohibitive costs of existing chloride sensors. To enable low cost diagnostic solutions, we recently established a citrate-derived synthesis platform for the development of new fluorescence sensors with high selectivity for chloride. As a next step, we herein designed a smartphone operated chloridometer that optimizes the analytical performance of the citrate-derived sensor materials for the detection of chloride in sweat. The sensor material demonstrated a wide linear range of 0.8–200 mM chloride and a diffusion-limited response time; sweat chloride levels corresponded to measurable changes in fluorescence emission that was captured by a smartphone. Clinical validation was performed with sweat from individuals with and without CF, demonstrating convenient sweat diagnostics with reliable detection of cystic fibrosis. To our knowledge, this is the first clinical study of a smartphone-based chloride sensor, paving the way for point-of-care diagnostic systems for CF.

UR - http://www.scopus.com/inward/record.url?scp=85020314566&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020314566&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2017.05.048

DO - 10.1016/j.bios.2017.05.048

M3 - Article

C2 - 28595077

AN - SCOPUS:85020314566

VL - 97

SP - 164

EP - 168

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

SN - 0956-5663

ER -