A solanum lycopersicum solanum pimpinellifolium linkage map of tomato displaying genomic locations of R-genes, RGAs, and candidate resistance/defense-response ESTs

Majid R. Foolad, Arun Sharma, Liping Zhang, David Nio-Liu, Hamid Ashrafi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We have identified an accession (LA2093) within the tomato wild species Solanum pimpinellifolium with many desirable characteristics, including biotic and abiotic stress tolerance and good fruit quality. To utilize the full genetic potential of LA2093 in tomato breeding, we have developed a linkage map based on an F 2 population of a cross between LA2093 and a tomato breeding line, using 115 RFLP, 94 EST, and 41 RGA markers. The map spanned 1002.4cM of the 12 tomato chromosomes with an average marker distance of 4.0cM. The length of the map and linear order of the markers were in good agreement with the published maps of tomato. The ESTs were chosen based on their sequence similarities with known resistance or defense-response genes, signal-transduction factors, transcriptional regulators, and genes encoding pathogenesis-related proteins. Locations of several ESTs and RGAs coincided with locations of several known tomato resistance genes and quantitative resistance loci (QRLs), suggesting that candidate-gene approach may be effective in identifying and mapping new R genes. This map will be useful for marker-assisted exploitation of desirable traits in LA2093 and other S. pimpinellifolium accessions, and possibly for utilization of genetic variation within S. lycopersicum.

Original languageEnglish (US)
Article number926090
JournalInternational Journal of Plant Genomics
Volume2008
DOIs
StatePublished - Dec 1 2008

Fingerprint

Solanum pimpinellifolium
vpr Genes
Solanum
Expressed Sequence Tags
Solanum lycopersicum
Lycopersicon esculentum
chromosome mapping
tomatoes
genomics
genes
Breeding
Genes
pathogenesis-related proteins
Chromosomes, Human, Pair 12
biotic stress
breeding lines
stress tolerance
abiotic stress
Regulator Genes
fruit quality

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science

Cite this

@article{09b85deded404529b49bd483a32977da,
title = "A solanum lycopersicum solanum pimpinellifolium linkage map of tomato displaying genomic locations of R-genes, RGAs, and candidate resistance/defense-response ESTs",
abstract = "We have identified an accession (LA2093) within the tomato wild species Solanum pimpinellifolium with many desirable characteristics, including biotic and abiotic stress tolerance and good fruit quality. To utilize the full genetic potential of LA2093 in tomato breeding, we have developed a linkage map based on an F 2 population of a cross between LA2093 and a tomato breeding line, using 115 RFLP, 94 EST, and 41 RGA markers. The map spanned 1002.4cM of the 12 tomato chromosomes with an average marker distance of 4.0cM. The length of the map and linear order of the markers were in good agreement with the published maps of tomato. The ESTs were chosen based on their sequence similarities with known resistance or defense-response genes, signal-transduction factors, transcriptional regulators, and genes encoding pathogenesis-related proteins. Locations of several ESTs and RGAs coincided with locations of several known tomato resistance genes and quantitative resistance loci (QRLs), suggesting that candidate-gene approach may be effective in identifying and mapping new R genes. This map will be useful for marker-assisted exploitation of desirable traits in LA2093 and other S. pimpinellifolium accessions, and possibly for utilization of genetic variation within S. lycopersicum.",
author = "Foolad, {Majid R.} and Arun Sharma and Liping Zhang and David Nio-Liu and Hamid Ashrafi",
year = "2008",
month = "12",
day = "1",
doi = "10.1155/2008/926090",
language = "English (US)",
volume = "2008",
journal = "International Journal of Plant Genomics",
issn = "1687-5370",
publisher = "Hindawi Publishing Corporation",

}

A solanum lycopersicum solanum pimpinellifolium linkage map of tomato displaying genomic locations of R-genes, RGAs, and candidate resistance/defense-response ESTs. / Foolad, Majid R.; Sharma, Arun; Zhang, Liping; Nio-Liu, David; Ashrafi, Hamid.

In: International Journal of Plant Genomics, Vol. 2008, 926090, 01.12.2008.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A solanum lycopersicum solanum pimpinellifolium linkage map of tomato displaying genomic locations of R-genes, RGAs, and candidate resistance/defense-response ESTs

AU - Foolad, Majid R.

AU - Sharma, Arun

AU - Zhang, Liping

AU - Nio-Liu, David

AU - Ashrafi, Hamid

PY - 2008/12/1

Y1 - 2008/12/1

N2 - We have identified an accession (LA2093) within the tomato wild species Solanum pimpinellifolium with many desirable characteristics, including biotic and abiotic stress tolerance and good fruit quality. To utilize the full genetic potential of LA2093 in tomato breeding, we have developed a linkage map based on an F 2 population of a cross between LA2093 and a tomato breeding line, using 115 RFLP, 94 EST, and 41 RGA markers. The map spanned 1002.4cM of the 12 tomato chromosomes with an average marker distance of 4.0cM. The length of the map and linear order of the markers were in good agreement with the published maps of tomato. The ESTs were chosen based on their sequence similarities with known resistance or defense-response genes, signal-transduction factors, transcriptional regulators, and genes encoding pathogenesis-related proteins. Locations of several ESTs and RGAs coincided with locations of several known tomato resistance genes and quantitative resistance loci (QRLs), suggesting that candidate-gene approach may be effective in identifying and mapping new R genes. This map will be useful for marker-assisted exploitation of desirable traits in LA2093 and other S. pimpinellifolium accessions, and possibly for utilization of genetic variation within S. lycopersicum.

AB - We have identified an accession (LA2093) within the tomato wild species Solanum pimpinellifolium with many desirable characteristics, including biotic and abiotic stress tolerance and good fruit quality. To utilize the full genetic potential of LA2093 in tomato breeding, we have developed a linkage map based on an F 2 population of a cross between LA2093 and a tomato breeding line, using 115 RFLP, 94 EST, and 41 RGA markers. The map spanned 1002.4cM of the 12 tomato chromosomes with an average marker distance of 4.0cM. The length of the map and linear order of the markers were in good agreement with the published maps of tomato. The ESTs were chosen based on their sequence similarities with known resistance or defense-response genes, signal-transduction factors, transcriptional regulators, and genes encoding pathogenesis-related proteins. Locations of several ESTs and RGAs coincided with locations of several known tomato resistance genes and quantitative resistance loci (QRLs), suggesting that candidate-gene approach may be effective in identifying and mapping new R genes. This map will be useful for marker-assisted exploitation of desirable traits in LA2093 and other S. pimpinellifolium accessions, and possibly for utilization of genetic variation within S. lycopersicum.

UR - http://www.scopus.com/inward/record.url?scp=62249088653&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=62249088653&partnerID=8YFLogxK

U2 - 10.1155/2008/926090

DO - 10.1155/2008/926090

M3 - Article

VL - 2008

JO - International Journal of Plant Genomics

JF - International Journal of Plant Genomics

SN - 1687-5370

M1 - 926090

ER -