A spectrin‐like protein from mouse brain membranes: Immunological and structural correlations with erythrocyte spectrin

Steven R. Goodman, Ian Zagon, Carol F. Whitfield, Linda A. Casoria, Patricia McLaughlin, Theresa L. Laskiewicz

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Membrane‐associated mouse brain spectrin is a 972,000 Mr, 10.5S, (αβ)2 tetramer containing two ∼ 240,000 Mr subunits and two ∼ 235,000 Mr subunits. Two‐dimensional [125I]tryptic peptide mapping indicates that these subunits share only limited and equivalent overlap with the α‐ and β‐subunits of red blood cell (RBC) spectrin. Both the 220,000 Mr β‐subunit of RBC spectrin and the 235,000 Mr β‐subunit of brain spectrin are phosphorylated in the intact mouse. In vitro analysis suggests that both are phosphorylated by a cAMP‐independent protein kinase. Antibodies against pure native mouse red blood cell spectrin cross‐react with brain spectrin, and antibodies against pure brain spectrin cross‐react with both the α‐and β‐subunits of mouse RBC spectrin. Both antibodies have been utilized to localize brain spectrin within distinct cellular entities of the mouse cerebellum. Granule cell neurons of the internal granule layer and Purkinje cell neurons demonstrated intense fluorscence of the cortical cytoplasm immediately adjacent to the plasma membrane and unstained nuclei, when either RBC or brain spectrin antibodies were utilized for staining. The molecular layer of the cerebellum stained only lightly, and oligodendrocytes and astrocytes appeared to have little fluorescence. Therefore, while brain is a tissue rich in nonerythroid spectrin, the concentration of these immunoreactive analogues is quite variable within distinct cellular entities of the cerebellum.

Original languageEnglish (US)
Pages (from-to)635-647
Number of pages13
JournalCell Motility
Volume3
Issue number5
DOIs
StatePublished - Jan 1 1983

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Spectrin
Erythrocytes
Membranes
Brain
Proteins
Cerebellum
Antibodies
Purkinje Cells
Peptide Mapping
Oligodendroglia
Astrocytes
Protein Kinases
Cytoplasm
Fluorescence
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Goodman, Steven R. ; Zagon, Ian ; Whitfield, Carol F. ; Casoria, Linda A. ; McLaughlin, Patricia ; Laskiewicz, Theresa L. / A spectrin‐like protein from mouse brain membranes : Immunological and structural correlations with erythrocyte spectrin. In: Cell Motility. 1983 ; Vol. 3, No. 5. pp. 635-647.
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A spectrin‐like protein from mouse brain membranes : Immunological and structural correlations with erythrocyte spectrin. / Goodman, Steven R.; Zagon, Ian; Whitfield, Carol F.; Casoria, Linda A.; McLaughlin, Patricia; Laskiewicz, Theresa L.

In: Cell Motility, Vol. 3, No. 5, 01.01.1983, p. 635-647.

Research output: Contribution to journalArticle

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