A straightforward NMR-spectroscopy-based method for rapid library screening

Aloysius H. Siriwardena, Fang Tian, Schroder Noble, James H. Prestegard

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

An exchange is better than a rest: An NMR screening method is described which facilitates the screening of compound mixtures for components that bind protein drug targets. The approach allows detection of active molecules thanks to a "probe" molecule, which competes with a library of potential ligands for binding sites on a targeted protein. The whole spectrum of active ligands, including those displaying either very fast or very slow exchange behavior, are equally susceptible to detection by this method.

Original languageEnglish (US)
Pages (from-to)3454-3457
Number of pages4
JournalAngewandte Chemie - International Edition
Volume41
Issue number18
DOIs
StatePublished - Sep 16 2002

Fingerprint

Nuclear magnetic resonance spectroscopy
Screening
Ligands
Proteins
Molecules
Binding sites
Binding Sites
Nuclear magnetic resonance
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

Cite this

Siriwardena, Aloysius H. ; Tian, Fang ; Noble, Schroder ; Prestegard, James H. / A straightforward NMR-spectroscopy-based method for rapid library screening. In: Angewandte Chemie - International Edition. 2002 ; Vol. 41, No. 18. pp. 3454-3457.
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A straightforward NMR-spectroscopy-based method for rapid library screening. / Siriwardena, Aloysius H.; Tian, Fang; Noble, Schroder; Prestegard, James H.

In: Angewandte Chemie - International Edition, Vol. 41, No. 18, 16.09.2002, p. 3454-3457.

Research output: Contribution to journalArticle

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