A structural model reveals energy transduction in dynein

Adrian W.R. Serohijos, Yiwen Chen, Feng Ding, Timothy C. Elston, Nikolay V. Dokholyan

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Intracellular active transport is driven by ATP-hydrolyzing motor proteins that move along cytoskeletal filaments. In particular, the microtubule- associated dynein motor is involved in the transport of organelles and vesicles, the maintenance of the Golgi, and mitosis. However, unlike kinesin and myosin, the mechanism by which dynein converts chemical energy into mechanical force remains largely a mystery, due primarily to the lack of a high-resolution molecular structure. Using homology modeling and normal mode analysis, we propose a complete atomic structure and a mechanism for force generation by the motor protein dynein. In agreement with very recent electron microscopy (EM) reconstructions showing dynein as a ring-shaped heptamer, our model consists of six ATPases of the AAA (ATPases associated with various cellular activities) superfamily and a C-terminal domain, which is experimentally known to control motor function. Our model shows a coiled coil spanning the diameter of the motor that accounts for previously unidentified structures in EM studies and provides a potential mechanism for long-range communication between the AAA domains. Furthermore, normal mode analysis reveals that the subunits of the motor that contain the nucleotide binding sites exhibit minimal movement, whereas the rest of the motor is very mobile. Our analysis suggests the likely domain rearrangements of the motor unit that generate its power stroke. This study provides insights into the structure and function of dynein that can guide further experimental investigations into energy transduction in dynein.

Original languageEnglish (US)
Pages (from-to)18540-18545
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number49
DOIs
StatePublished - Dec 5 2006

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Dyneins
Structural Models
Adenosine Triphosphatases
Electron Microscopy
Kinesin
Transport Vesicles
Active Biological Transport
Myosins
Molecular Structure
Cytoskeleton
Mitosis
Microtubules
Organelles
Proteins
Nucleotides
Adenosine Triphosphate
Stroke
Binding Sites
Maintenance

All Science Journal Classification (ASJC) codes

  • General

Cite this

Serohijos, Adrian W.R. ; Chen, Yiwen ; Ding, Feng ; Elston, Timothy C. ; Dokholyan, Nikolay V. / A structural model reveals energy transduction in dynein. In: Proceedings of the National Academy of Sciences of the United States of America. 2006 ; Vol. 103, No. 49. pp. 18540-18545.
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A structural model reveals energy transduction in dynein. / Serohijos, Adrian W.R.; Chen, Yiwen; Ding, Feng; Elston, Timothy C.; Dokholyan, Nikolay V.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 49, 05.12.2006, p. 18540-18545.

Research output: Contribution to journalArticle

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