A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus

R. V. Sairam, G. Franklin, R. Hassel, B. Smith, K. Meeker, N. Kashikar, M. Parani, D. Al Abed, S. Ismail, K. Berry, S. L. Goldman

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

We have developed an efficient protocol for callus induction and plant regeneration in three elite soybean cultivars (Williams 82, Loda and Newton). The technique is most novel in that the shoot buds developed from the nodal callus. Callus induction and subsequent shoot bud differentiation were achieved from the proximal end of cotyledonary explants on modified Murashige and Skoog (MS) media containing 2.26 μM 2,4-dichlorophenoxy-acetic acid (2,4-D) and 8.8 μM benzyladenine (BAP), respectively. Varying the carbon source optimized the regeneration system further. Among the various carbon sources tested, sorbitol was found to be the best for callus induction and maltose for plant regeneration.

Original languageEnglish (US)
Pages (from-to)79-85
Number of pages7
JournalPlant Cell, Tissue and Organ Culture
Volume75
Issue number1
DOIs
StatePublished - Oct 1 2003

All Science Journal Classification (ASJC) codes

  • Horticulture

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