A transcriptome-based genetic map of Chinese chestnut (Castanea mollissima) and identification of regions of segmental homology with peach (Prunus persica)

T. L. Kubisiak, C. D. Nelson, M. E. Staton, T. Zhebentyayeva, C. Smith, B. A. Olukolu, G. C. Fang, F. V. Hebard, S. Anagnostakis, N. Wheeler, P. H. Sisco, A. G. Abbott, R. R. Sederoff

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The Chinese chestnut (Castanea mollissima) carries resistance to Cryphonectria parasitica, the fungal pathogen inciting chestnut blight. The pathogen, introduced from Asia, devastated the American chestnut (Castanea dentata) throughout its native range early in the twentieth century. A highly informative genetic map of Chinese chestnut was constructed to extend genomic studies in the Fagaceae and to aid the introgression of Chinese chestnut blight resistance genes into American chestnut. Two mapping populations were established with three Chinese chestnut parents, 'Mahogany', 'Nanking', and 'Vanuxem', totaling 337 progeny. The transcriptome-based genetic map was created with 329 simple sequence repeat and 1,064 single nucleotide polymorphism markers all derived from expressed sequence tag sequences. Genetic maps for each parent were developed and combined to establish 12 consensus linkage groups spanning 742 cM, providing the the most comprehensive genetic map for a Fagaceae species to date. Over 75 % of the mapped markers from the Chinese chestnut consensus genetic map were placed on the physical map using overgo hybridization, providing a fully integrated genetic and physical map resource for Castanea spp. About half (57 %) of the Chinese chestnut genetic map could be assigned to regions of segmental homology with 58 % of the peach (Prunus persica) genome assembly. A three quantitative trait loci (QTL) model for blight resistance was verified using the new genetic markers and an existing interspecies (C. mollissima × C. dentata) F2 mapping population. Two of the blight resistance QTLs in chestnut shared synteny with two QTLs for powdery mildew resistance in peach, indicating the potential conservation of disease resistance genes at these loci.

Original languageEnglish (US)
Pages (from-to)557-571
Number of pages15
JournalTree Genetics and Genomes
Volume9
Issue number2
DOIs
StatePublished - Jan 1 2013

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Castanea mollissima
Fagaceae
Prunus persica
homology
Transcriptome
transcriptome
peaches
Castanea dentata
Cryphonectria parasitica
Synteny
Disease Resistance
Quantitative Trait Loci
Expressed Sequence Tags
Genetic Markers
quantitative trait loci
Microsatellite Repeats
Population
Genes
Single Nucleotide Polymorphism
physical chromosome mapping

All Science Journal Classification (ASJC) codes

  • Forestry
  • Molecular Biology
  • Genetics
  • Horticulture

Cite this

Kubisiak, T. L. ; Nelson, C. D. ; Staton, M. E. ; Zhebentyayeva, T. ; Smith, C. ; Olukolu, B. A. ; Fang, G. C. ; Hebard, F. V. ; Anagnostakis, S. ; Wheeler, N. ; Sisco, P. H. ; Abbott, A. G. ; Sederoff, R. R. / A transcriptome-based genetic map of Chinese chestnut (Castanea mollissima) and identification of regions of segmental homology with peach (Prunus persica). In: Tree Genetics and Genomes. 2013 ; Vol. 9, No. 2. pp. 557-571.
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abstract = "The Chinese chestnut (Castanea mollissima) carries resistance to Cryphonectria parasitica, the fungal pathogen inciting chestnut blight. The pathogen, introduced from Asia, devastated the American chestnut (Castanea dentata) throughout its native range early in the twentieth century. A highly informative genetic map of Chinese chestnut was constructed to extend genomic studies in the Fagaceae and to aid the introgression of Chinese chestnut blight resistance genes into American chestnut. Two mapping populations were established with three Chinese chestnut parents, 'Mahogany', 'Nanking', and 'Vanuxem', totaling 337 progeny. The transcriptome-based genetic map was created with 329 simple sequence repeat and 1,064 single nucleotide polymorphism markers all derived from expressed sequence tag sequences. Genetic maps for each parent were developed and combined to establish 12 consensus linkage groups spanning 742 cM, providing the the most comprehensive genetic map for a Fagaceae species to date. Over 75 {\%} of the mapped markers from the Chinese chestnut consensus genetic map were placed on the physical map using overgo hybridization, providing a fully integrated genetic and physical map resource for Castanea spp. About half (57 {\%}) of the Chinese chestnut genetic map could be assigned to regions of segmental homology with 58 {\%} of the peach (Prunus persica) genome assembly. A three quantitative trait loci (QTL) model for blight resistance was verified using the new genetic markers and an existing interspecies (C. mollissima × C. dentata) F2 mapping population. Two of the blight resistance QTLs in chestnut shared synteny with two QTLs for powdery mildew resistance in peach, indicating the potential conservation of disease resistance genes at these loci.",
author = "Kubisiak, {T. L.} and Nelson, {C. D.} and Staton, {M. E.} and T. Zhebentyayeva and C. Smith and Olukolu, {B. A.} and Fang, {G. C.} and Hebard, {F. V.} and S. Anagnostakis and N. Wheeler and Sisco, {P. H.} and Abbott, {A. G.} and Sederoff, {R. R.}",
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Kubisiak, TL, Nelson, CD, Staton, ME, Zhebentyayeva, T, Smith, C, Olukolu, BA, Fang, GC, Hebard, FV, Anagnostakis, S, Wheeler, N, Sisco, PH, Abbott, AG & Sederoff, RR 2013, 'A transcriptome-based genetic map of Chinese chestnut (Castanea mollissima) and identification of regions of segmental homology with peach (Prunus persica)', Tree Genetics and Genomes, vol. 9, no. 2, pp. 557-571. https://doi.org/10.1007/s11295-012-0579-3

A transcriptome-based genetic map of Chinese chestnut (Castanea mollissima) and identification of regions of segmental homology with peach (Prunus persica). / Kubisiak, T. L.; Nelson, C. D.; Staton, M. E.; Zhebentyayeva, T.; Smith, C.; Olukolu, B. A.; Fang, G. C.; Hebard, F. V.; Anagnostakis, S.; Wheeler, N.; Sisco, P. H.; Abbott, A. G.; Sederoff, R. R.

In: Tree Genetics and Genomes, Vol. 9, No. 2, 01.01.2013, p. 557-571.

Research output: Contribution to journalArticle

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T1 - A transcriptome-based genetic map of Chinese chestnut (Castanea mollissima) and identification of regions of segmental homology with peach (Prunus persica)

AU - Kubisiak, T. L.

AU - Nelson, C. D.

AU - Staton, M. E.

AU - Zhebentyayeva, T.

AU - Smith, C.

AU - Olukolu, B. A.

AU - Fang, G. C.

AU - Hebard, F. V.

AU - Anagnostakis, S.

AU - Wheeler, N.

AU - Sisco, P. H.

AU - Abbott, A. G.

AU - Sederoff, R. R.

PY - 2013/1/1

Y1 - 2013/1/1

N2 - The Chinese chestnut (Castanea mollissima) carries resistance to Cryphonectria parasitica, the fungal pathogen inciting chestnut blight. The pathogen, introduced from Asia, devastated the American chestnut (Castanea dentata) throughout its native range early in the twentieth century. A highly informative genetic map of Chinese chestnut was constructed to extend genomic studies in the Fagaceae and to aid the introgression of Chinese chestnut blight resistance genes into American chestnut. Two mapping populations were established with three Chinese chestnut parents, 'Mahogany', 'Nanking', and 'Vanuxem', totaling 337 progeny. The transcriptome-based genetic map was created with 329 simple sequence repeat and 1,064 single nucleotide polymorphism markers all derived from expressed sequence tag sequences. Genetic maps for each parent were developed and combined to establish 12 consensus linkage groups spanning 742 cM, providing the the most comprehensive genetic map for a Fagaceae species to date. Over 75 % of the mapped markers from the Chinese chestnut consensus genetic map were placed on the physical map using overgo hybridization, providing a fully integrated genetic and physical map resource for Castanea spp. About half (57 %) of the Chinese chestnut genetic map could be assigned to regions of segmental homology with 58 % of the peach (Prunus persica) genome assembly. A three quantitative trait loci (QTL) model for blight resistance was verified using the new genetic markers and an existing interspecies (C. mollissima × C. dentata) F2 mapping population. Two of the blight resistance QTLs in chestnut shared synteny with two QTLs for powdery mildew resistance in peach, indicating the potential conservation of disease resistance genes at these loci.

AB - The Chinese chestnut (Castanea mollissima) carries resistance to Cryphonectria parasitica, the fungal pathogen inciting chestnut blight. The pathogen, introduced from Asia, devastated the American chestnut (Castanea dentata) throughout its native range early in the twentieth century. A highly informative genetic map of Chinese chestnut was constructed to extend genomic studies in the Fagaceae and to aid the introgression of Chinese chestnut blight resistance genes into American chestnut. Two mapping populations were established with three Chinese chestnut parents, 'Mahogany', 'Nanking', and 'Vanuxem', totaling 337 progeny. The transcriptome-based genetic map was created with 329 simple sequence repeat and 1,064 single nucleotide polymorphism markers all derived from expressed sequence tag sequences. Genetic maps for each parent were developed and combined to establish 12 consensus linkage groups spanning 742 cM, providing the the most comprehensive genetic map for a Fagaceae species to date. Over 75 % of the mapped markers from the Chinese chestnut consensus genetic map were placed on the physical map using overgo hybridization, providing a fully integrated genetic and physical map resource for Castanea spp. About half (57 %) of the Chinese chestnut genetic map could be assigned to regions of segmental homology with 58 % of the peach (Prunus persica) genome assembly. A three quantitative trait loci (QTL) model for blight resistance was verified using the new genetic markers and an existing interspecies (C. mollissima × C. dentata) F2 mapping population. Two of the blight resistance QTLs in chestnut shared synteny with two QTLs for powdery mildew resistance in peach, indicating the potential conservation of disease resistance genes at these loci.

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