Abstract
Plasmodium falciparum is the pathogenic agent of the most lethal of human malarias. Transgenic P. falciparum parasites expressing luciferase have been created to study drug interventions of both asexual and sexual blood stages but luciferase-expressing mosquito stage and liver stage parasites have not been created which has prevented the easy quantification of mosquito stage development (e.g. for transmission blocking interventions) and liver stage development (for interventions that prevent infection). To overcome this obstacle, we have created a transgenic P. falciparum NF54 parasite that expresses a GFP-luciferase transgene throughout the life cycle. Luciferase expression is robust and measurable at all life cycle stages, including midgut oocyst, salivary gland sporozoites and liver stages, where in vivo development is easily measurable using humanized mouse infections in conjunction with an in vivo imaging system. This parasite reporter strain will accelerate testing of interventions against pre-erythrocytic life cycle stages.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 143-147 |
| Number of pages | 5 |
| Journal | Molecular and biochemical parasitology |
| Volume | 186 |
| Issue number | 2 |
| DOIs | |
| State | Published - Dec 1 2012 |
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All Science Journal Classification (ASJC) codes
- Parasitology
- Molecular Biology
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A transgenic Plasmodium falciparum NF54 strain that expresses GFP-luciferase throughout the parasite life cycle. / Vaughan, Ashley M.; Mikolajczak, Sebastian A.; Camargo, Nelly; Lakshmanan, Viswanathan; Kennedy, Mark; Lindner, Scott Eugene; Miller, Jessica L.; Hume, Jen C.C.; Kappe, Stefan H.I.
In: Molecular and biochemical parasitology, Vol. 186, No. 2, 01.12.2012, p. 143-147.Research output: Contribution to journal › Article
TY - JOUR
T1 - A transgenic Plasmodium falciparum NF54 strain that expresses GFP-luciferase throughout the parasite life cycle
AU - Vaughan, Ashley M.
AU - Mikolajczak, Sebastian A.
AU - Camargo, Nelly
AU - Lakshmanan, Viswanathan
AU - Kennedy, Mark
AU - Lindner, Scott Eugene
AU - Miller, Jessica L.
AU - Hume, Jen C.C.
AU - Kappe, Stefan H.I.
PY - 2012/12/1
Y1 - 2012/12/1
N2 - Plasmodium falciparum is the pathogenic agent of the most lethal of human malarias. Transgenic P. falciparum parasites expressing luciferase have been created to study drug interventions of both asexual and sexual blood stages but luciferase-expressing mosquito stage and liver stage parasites have not been created which has prevented the easy quantification of mosquito stage development (e.g. for transmission blocking interventions) and liver stage development (for interventions that prevent infection). To overcome this obstacle, we have created a transgenic P. falciparum NF54 parasite that expresses a GFP-luciferase transgene throughout the life cycle. Luciferase expression is robust and measurable at all life cycle stages, including midgut oocyst, salivary gland sporozoites and liver stages, where in vivo development is easily measurable using humanized mouse infections in conjunction with an in vivo imaging system. This parasite reporter strain will accelerate testing of interventions against pre-erythrocytic life cycle stages.
AB - Plasmodium falciparum is the pathogenic agent of the most lethal of human malarias. Transgenic P. falciparum parasites expressing luciferase have been created to study drug interventions of both asexual and sexual blood stages but luciferase-expressing mosquito stage and liver stage parasites have not been created which has prevented the easy quantification of mosquito stage development (e.g. for transmission blocking interventions) and liver stage development (for interventions that prevent infection). To overcome this obstacle, we have created a transgenic P. falciparum NF54 parasite that expresses a GFP-luciferase transgene throughout the life cycle. Luciferase expression is robust and measurable at all life cycle stages, including midgut oocyst, salivary gland sporozoites and liver stages, where in vivo development is easily measurable using humanized mouse infections in conjunction with an in vivo imaging system. This parasite reporter strain will accelerate testing of interventions against pre-erythrocytic life cycle stages.
UR - http://www.scopus.com/inward/record.url?scp=84870405599&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84870405599&partnerID=8YFLogxK
U2 - 10.1016/j.molbiopara.2012.10.004
DO - 10.1016/j.molbiopara.2012.10.004
M3 - Article
C2 - 23107927
AN - SCOPUS:84870405599
VL - 186
SP - 143
EP - 147
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
SN - 0166-6851
IS - 2
ER -