A transgenic Plasmodium falciparum NF54 strain that expresses GFP-luciferase throughout the parasite life cycle

Ashley M. Vaughan, Sebastian A. Mikolajczak, Nelly Camargo, Viswanathan Lakshmanan, Mark Kennedy, Scott E. Lindner, Jessica L. Miller, Jen C.C. Hume, Stefan H.I. Kappe

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Plasmodium falciparum is the pathogenic agent of the most lethal of human malarias. Transgenic P. falciparum parasites expressing luciferase have been created to study drug interventions of both asexual and sexual blood stages but luciferase-expressing mosquito stage and liver stage parasites have not been created which has prevented the easy quantification of mosquito stage development (e.g. for transmission blocking interventions) and liver stage development (for interventions that prevent infection). To overcome this obstacle, we have created a transgenic P. falciparum NF54 parasite that expresses a GFP-luciferase transgene throughout the life cycle. Luciferase expression is robust and measurable at all life cycle stages, including midgut oocyst, salivary gland sporozoites and liver stages, where in vivo development is easily measurable using humanized mouse infections in conjunction with an in vivo imaging system. This parasite reporter strain will accelerate testing of interventions against pre-erythrocytic life cycle stages.

Original languageEnglish (US)
Pages (from-to)143-147
Number of pages5
JournalMolecular and biochemical parasitology
Volume186
Issue number2
DOIs
StatePublished - Dec 1 2012

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Molecular Biology

Cite this

Vaughan, A. M., Mikolajczak, S. A., Camargo, N., Lakshmanan, V., Kennedy, M., Lindner, S. E., Miller, J. L., Hume, J. C. C., & Kappe, S. H. I. (2012). A transgenic Plasmodium falciparum NF54 strain that expresses GFP-luciferase throughout the parasite life cycle. Molecular and biochemical parasitology, 186(2), 143-147. https://doi.org/10.1016/j.molbiopara.2012.10.004