A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex

Kerry O'Donnell, Cécile Gueidan, Stacy Sink, Peter R. Johnston, Pedro W. Crous, Anthony Glenn, Ron Riley, Nicholas C. Zitomer, Patrick Colyer, Cees Waalwijk, Theo van der Lee, Antonio Moretti, Seogchan Kang, Hye Seon Kim, David Michael Geiser, Jean H. Juba, Robert P. Baayen, Matthew G. Cromey, Sean Bithell, Deanna A. SuttonKerstin Skovgaard, Randy Ploetz, H. Corby Kistler, Monica Elliott, Mike Davis, Brice A.J. Sarver

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

We constructed a two-locus database, comprising partial translation elongation factor (EF-1α) gene sequences and nearly full-length sequences of the nuclear ribosomal intergenic spacer region (IGS rDNA) for 850 isolates spanning the phylogenetic breadth of the Fusarium oxysporum species complex (FOSC). Of the 850 isolates typed, 101 EF-1α, 203 IGS rDNA, and 256 two-locus sequence types (STs) were differentiated. Analysis of the combined dataset suggests that two-thirds of the STs might be associated with a single host plant. This analysis also revealed that the 26 STs associated with human mycoses were genetically diverse, including several which appear to be nosocomial in origin. A congruence analysis, comparing partial EF-1α and IGS rDNA bootstrap consensus, identified a significant number of conflicting relationships dispersed throughout the bipartitions, suggesting that some of the IGS rDNA sequences may be non-orthologous. We also evaluated enniatin, fumonisin and moniliformin mycotoxin production in vitro within a phylogenetic framework.

Original languageEnglish (US)
Pages (from-to)936-948
Number of pages13
JournalFungal Genetics and Biology
Volume46
Issue number12
DOIs
StatePublished - Dec 1 2009

Fingerprint

Peptide Elongation Factor 1
Nucleic Acid Databases
Fusarium
Ribosomal DNA
Fumonisins
Intergenic DNA
Mycoses
Mycotoxins
Databases
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Genetics

Cite this

O'Donnell, K., Gueidan, C., Sink, S., Johnston, P. R., Crous, P. W., Glenn, A., ... Sarver, B. A. J. (2009). A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex. Fungal Genetics and Biology, 46(12), 936-948. https://doi.org/10.1016/j.fgb.2009.08.006
O'Donnell, Kerry ; Gueidan, Cécile ; Sink, Stacy ; Johnston, Peter R. ; Crous, Pedro W. ; Glenn, Anthony ; Riley, Ron ; Zitomer, Nicholas C. ; Colyer, Patrick ; Waalwijk, Cees ; Lee, Theo van der ; Moretti, Antonio ; Kang, Seogchan ; Kim, Hye Seon ; Geiser, David Michael ; Juba, Jean H. ; Baayen, Robert P. ; Cromey, Matthew G. ; Bithell, Sean ; Sutton, Deanna A. ; Skovgaard, Kerstin ; Ploetz, Randy ; Corby Kistler, H. ; Elliott, Monica ; Davis, Mike ; Sarver, Brice A.J. / A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex. In: Fungal Genetics and Biology. 2009 ; Vol. 46, No. 12. pp. 936-948.
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O'Donnell, K, Gueidan, C, Sink, S, Johnston, PR, Crous, PW, Glenn, A, Riley, R, Zitomer, NC, Colyer, P, Waalwijk, C, Lee, TVD, Moretti, A, Kang, S, Kim, HS, Geiser, DM, Juba, JH, Baayen, RP, Cromey, MG, Bithell, S, Sutton, DA, Skovgaard, K, Ploetz, R, Corby Kistler, H, Elliott, M, Davis, M & Sarver, BAJ 2009, 'A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex', Fungal Genetics and Biology, vol. 46, no. 12, pp. 936-948. https://doi.org/10.1016/j.fgb.2009.08.006

A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex. / O'Donnell, Kerry; Gueidan, Cécile; Sink, Stacy; Johnston, Peter R.; Crous, Pedro W.; Glenn, Anthony; Riley, Ron; Zitomer, Nicholas C.; Colyer, Patrick; Waalwijk, Cees; Lee, Theo van der; Moretti, Antonio; Kang, Seogchan; Kim, Hye Seon; Geiser, David Michael; Juba, Jean H.; Baayen, Robert P.; Cromey, Matthew G.; Bithell, Sean; Sutton, Deanna A.; Skovgaard, Kerstin; Ploetz, Randy; Corby Kistler, H.; Elliott, Monica; Davis, Mike; Sarver, Brice A.J.

In: Fungal Genetics and Biology, Vol. 46, No. 12, 01.12.2009, p. 936-948.

Research output: Contribution to journalArticle

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T1 - A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex

AU - O'Donnell, Kerry

AU - Gueidan, Cécile

AU - Sink, Stacy

AU - Johnston, Peter R.

AU - Crous, Pedro W.

AU - Glenn, Anthony

AU - Riley, Ron

AU - Zitomer, Nicholas C.

AU - Colyer, Patrick

AU - Waalwijk, Cees

AU - Lee, Theo van der

AU - Moretti, Antonio

AU - Kang, Seogchan

AU - Kim, Hye Seon

AU - Geiser, David Michael

AU - Juba, Jean H.

AU - Baayen, Robert P.

AU - Cromey, Matthew G.

AU - Bithell, Sean

AU - Sutton, Deanna A.

AU - Skovgaard, Kerstin

AU - Ploetz, Randy

AU - Corby Kistler, H.

AU - Elliott, Monica

AU - Davis, Mike

AU - Sarver, Brice A.J.

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