A Unique Arabinose 5-Phosphate Isomerase Found within a Genomic Island Associated with the Uropathogenicity of Escherichia coli CFT073

Joshua A. Mosberg, Alejandra Yep, Timothy Charles Meredith, Sara Smith, Pan Fen Wang, Harry L.T. Mobley, Ronald W. Woodard

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Previous studies showed that deletion of genes c3405 to c3410 from PAI-metV, a genomic island from Escherichia coli CFT073, results in a strain that fails to compete with wild-type CFT073 after a transurethral cochallenge in mice and is deficient in the ability to independently colonize the mouse kidney. Our analysis of c3405 to c3410 suggests that these genes constitute an operon with a role in the internalization and utilization of an unknown carbohydrate. This operon is not found in E. coli K-12 but is present in a small number of pathogenic E. coli and Shigella boydii strains. One of the genes, c3406, encodes a protein with significant homology to the sugar isomerase domain of arabinose 5-phosphate isomerases but lacking the tandem cystathionine beta-synthase domains found in the other arabinose 5-phosphate isomerases of E. coli. We prepared recombinant c3406 protein, found it to possess arabinose 5-phosphate isomerase activity, and characterized this activity in detail. We also constructed a c3406 deletion mutant of E. coli CFT073 and demonstrated that this deletion mutant was still able to compete with wild-type CFT073 in a transurethral cochallenge in mice and could colonize the mouse kidney. These results demonstrate that the presence of c3406 is not essential for a pathogenic phenotype.

Original languageEnglish (US)
Pages (from-to)2981-2988
Number of pages8
JournalJournal of bacteriology
Volume193
Issue number12
DOIs
StatePublished - Jun 1 2011

Fingerprint

Genomic Islands
Escherichia coli
Operon
Shigella boydii
Cystathionine beta-Synthase
Kidney
Isomerases
Gene Deletion
Recombinant Proteins
Genes
Carbohydrates
arabinose-5-phosphate isomerase
Phenotype
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Mosberg, Joshua A. ; Yep, Alejandra ; Meredith, Timothy Charles ; Smith, Sara ; Wang, Pan Fen ; Mobley, Harry L.T. ; Woodard, Ronald W. / A Unique Arabinose 5-Phosphate Isomerase Found within a Genomic Island Associated with the Uropathogenicity of Escherichia coli CFT073. In: Journal of bacteriology. 2011 ; Vol. 193, No. 12. pp. 2981-2988.
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A Unique Arabinose 5-Phosphate Isomerase Found within a Genomic Island Associated with the Uropathogenicity of Escherichia coli CFT073. / Mosberg, Joshua A.; Yep, Alejandra; Meredith, Timothy Charles; Smith, Sara; Wang, Pan Fen; Mobley, Harry L.T.; Woodard, Ronald W.

In: Journal of bacteriology, Vol. 193, No. 12, 01.06.2011, p. 2981-2988.

Research output: Contribution to journalArticle

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