A unique population of cd34+ cells in cord blood

Maya T. Nimgaonkar, Rochelle A. Roscoe, Jeannette Persichetti, Witold Rybka, Alan Winkelstein, Edward D. Ball

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Human umbilical cord blood (CB) is a rich source of hematopoietic stem cells for both research and stem cell transplantation. In clinical studies, it appears that recovery from myeloablative therapy using CB requires significantly fewer cells than a typical allogeneic marrow transplant. This suggests that CB may be enriched for early hematopoietic progenitors. The present studies were undertaken to determine the presence of CD34+ cells in CB with the phenotypic characteristics of multi‐potential stem cells. In 22 CB harvests, the average percentage of CD34+ cells was 1.33 ± 0.21% (SE), a value similar to that in adult normal bone marrows (BM). However, the distribution of CD34+ cells was distinctly different from either BM or granulocyte colony‐stimulating factor (G‐CSF) mobilized peripheral blood stem cell harvests. CB contained a defined population of brightly staining CD34+ cells with low side scatter. These CD34 (bright) cells comprised a mean of 14.5 ± 2.5% of the CB CD34+ cells, whereas <1% of BM CD34+ cells has been shown to be CD34‐bright. Eighty‐five to ninety percent were negative for three antigens expressed at an early stage of stem cell maturation: CD38, HLA‐DR and LFA‐1. Fifty‐five percent of these CD34 (bright) cells did not express the CD45RA isoform, an additional marker of immaturity. The antigen‐bright cells also lacked lineage‐specific antigens including CD33, CD56, CD19, CD10 and CD7 as well as CD71. Approximately 46% were Thy‐1+, and 40% expressed c‐kit receptors. These data suggest that, by phenotypic criteria, CB may be a particularly enriched source of primitive hematopoietic precursors.

Original languageEnglish (US)
Pages (from-to)158-166
Number of pages9
JournalSTEM CELLS
Volume13
Issue number2
DOIs
StatePublished - Jan 1 1995

Fingerprint

Fetal Blood
Population
Bone Marrow
Sialic Acid Binding Ig-like Lectin 3
Stem Cells
Stem Cell Research
Stem Cell Transplantation
Hematopoietic Stem Cells
Granulocytes
Bone Marrow Cells
Blood Cells
Protein Isoforms
Staining and Labeling
Transplants
Antigens

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Nimgaonkar, M. T., Roscoe, R. A., Persichetti, J., Rybka, W., Winkelstein, A., & Ball, E. D. (1995). A unique population of cd34+ cells in cord blood. STEM CELLS, 13(2), 158-166. https://doi.org/10.1002/stem.5530130207
Nimgaonkar, Maya T. ; Roscoe, Rochelle A. ; Persichetti, Jeannette ; Rybka, Witold ; Winkelstein, Alan ; Ball, Edward D. / A unique population of cd34+ cells in cord blood. In: STEM CELLS. 1995 ; Vol. 13, No. 2. pp. 158-166.
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Nimgaonkar, MT, Roscoe, RA, Persichetti, J, Rybka, W, Winkelstein, A & Ball, ED 1995, 'A unique population of cd34+ cells in cord blood', STEM CELLS, vol. 13, no. 2, pp. 158-166. https://doi.org/10.1002/stem.5530130207

A unique population of cd34+ cells in cord blood. / Nimgaonkar, Maya T.; Roscoe, Rochelle A.; Persichetti, Jeannette; Rybka, Witold; Winkelstein, Alan; Ball, Edward D.

In: STEM CELLS, Vol. 13, No. 2, 01.01.1995, p. 158-166.

Research output: Contribution to journalArticle

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Nimgaonkar MT, Roscoe RA, Persichetti J, Rybka W, Winkelstein A, Ball ED. A unique population of cd34+ cells in cord blood. STEM CELLS. 1995 Jan 1;13(2):158-166. https://doi.org/10.1002/stem.5530130207