A versatile click-compatible monolignol probe to study lignin deposition in plant cell walls

Jyotsna L. Pandey, Bo Wang, Brett G. Diehl, Thomas Lehman Richard, Gong Chen, Charles T. Anderson

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Lignin plays important structural and functional roles in plants by forming a hydrophobic matrix in secondary cell walls that enhances mechanical strength and resists microbial decay. While the importance of the lignin matrix is well documented and the biosynthetic pathways for monolignols are known, the process by which lignin precursors or monolignols are transported and polymerized to form this matrix remains a subject of considerable debate. In this study, we have synthesized and tested an analog of coniferyl alcohol that has been modified to contain an ethynyl group at the C-3 position. This modification enables fluorescent tagging and imaging of this molecule after its incorporation into plant tissue by click chemistry-assisted covalent labeling with a fluorescent azide dye, and confers a distinct Raman signature that could be used for Raman imaging. We found that this monolignol analog is incorporated into in vitro-polymerized dehydrogenation polymer (DHP) lignin and into root epidermal cell walls of 4-day-old Arabidopsis seedlings. Incorporation of the analog in stem sections of 6-week-old Arabidopsis thaliana plants and labeling with an Alexa-594 azide dye revealed the precise locations of new lignin polymerization. Results from this study indicate that this molecule can provide high-resolution localization of lignification during plant cell wall maturation and lignin matrix assembly.

Original languageEnglish (US)
Article numbere0121334
JournalPLoS One
Volume10
Issue number4
DOIs
StatePublished - Apr 17 2015

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Lignin
Plant Cells
Cell Wall
probes (equipment)
lignin
cell walls
azides
Azides
Arabidopsis
Labeling
dyes
Coloring Agents
Cells
image analysis
Click Chemistry
coniferyl alcohol
Imaging techniques
Molecules
lignification
Biosynthetic Pathways

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

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abstract = "Lignin plays important structural and functional roles in plants by forming a hydrophobic matrix in secondary cell walls that enhances mechanical strength and resists microbial decay. While the importance of the lignin matrix is well documented and the biosynthetic pathways for monolignols are known, the process by which lignin precursors or monolignols are transported and polymerized to form this matrix remains a subject of considerable debate. In this study, we have synthesized and tested an analog of coniferyl alcohol that has been modified to contain an ethynyl group at the C-3 position. This modification enables fluorescent tagging and imaging of this molecule after its incorporation into plant tissue by click chemistry-assisted covalent labeling with a fluorescent azide dye, and confers a distinct Raman signature that could be used for Raman imaging. We found that this monolignol analog is incorporated into in vitro-polymerized dehydrogenation polymer (DHP) lignin and into root epidermal cell walls of 4-day-old Arabidopsis seedlings. Incorporation of the analog in stem sections of 6-week-old Arabidopsis thaliana plants and labeling with an Alexa-594 azide dye revealed the precise locations of new lignin polymerization. Results from this study indicate that this molecule can provide high-resolution localization of lignification during plant cell wall maturation and lignin matrix assembly.",
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A versatile click-compatible monolignol probe to study lignin deposition in plant cell walls. / Pandey, Jyotsna L.; Wang, Bo; Diehl, Brett G.; Richard, Thomas Lehman; Chen, Gong; Anderson, Charles T.

In: PLoS One, Vol. 10, No. 4, e0121334, 17.04.2015.

Research output: Contribution to journalArticle

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