A visual screen of a GFP-fusion library identifies a new type of nuclear envelope membrane protein

Melissa Rolls, Pascal A. Stein, Stephen S. Taylor, Edward Ha, Frank McKeon, Tom A. Rapoport

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

The nuclear envelope (NE) is a distinct subdomain of the ER, but few membrane components have been described that are specific to it. We performed a visual screen in tissue culture cells to identify proteins targeted to the NE. This approach does not require assumptions about the nature of the association with the NE or the physical separation of NE and ER. We confirmed that screening a library of fusions to the green fluorescent protein can be used to identify proteins targeted to various subcompartments of mammalian cells, including the NE. With this approach, we identified a new NE membrane protein, named nurim. Nurim is a multispanning membrane protein without large hydrophilic domains that is very tightly associated with the nucleus. Unlike the known NE membrane proteins, it is neither associated with nuclear pores, nor targeted like lamin-associated membrane proteins. Thus, nurim is a new type of NE membrane protein that is localized to the NE by a distinct mechanism.

Original languageEnglish (US)
Pages (from-to)29-43
Number of pages15
JournalJournal of Cell Biology
Volume146
Issue number1
DOIs
StatePublished - Jul 12 1999

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Nuclear Envelope
Libraries
Membrane Proteins
Lamins
Nuclear Pore
Green Fluorescent Proteins
Proteins
Cell Culture Techniques

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Rolls, Melissa ; Stein, Pascal A. ; Taylor, Stephen S. ; Ha, Edward ; McKeon, Frank ; Rapoport, Tom A. / A visual screen of a GFP-fusion library identifies a new type of nuclear envelope membrane protein. In: Journal of Cell Biology. 1999 ; Vol. 146, No. 1. pp. 29-43.
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A visual screen of a GFP-fusion library identifies a new type of nuclear envelope membrane protein. / Rolls, Melissa; Stein, Pascal A.; Taylor, Stephen S.; Ha, Edward; McKeon, Frank; Rapoport, Tom A.

In: Journal of Cell Biology, Vol. 146, No. 1, 12.07.1999, p. 29-43.

Research output: Contribution to journalArticle

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