A1, a Bcl-2 family member, prolongs cell survival and permits myeloid differentiation

Elaine Y. Lin, Amos Orlofsky, Hong Gang Wang, John C. Reed, Michael B. Prystowsky

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

A1, a bcl-2 family member, has been identified as a hematopoietic- specific, early inducible gene. In this study it is shown that stable transfection of A1 into an interleukin-3 (IL-3)dependent myeloid precursor cell line, 32D cl3, leads to a retardation of IL-3 withdrawal-induced cell death similar to that observed with transfection of bcl-2. However, unlike bcl-2, A1 expression permits the accumulation of differentiated myeloid cells both before and after IL-3 withdrawal. Total cell accumulation, on the other hand, is considerably greater after IL-3 deprivation in the bcl-2 transfectant than in A1-expressing cells. Cells cotransfected with the two genes behave similarly to cells singly transfected with bcl-2, except that viability following IL-3 withdrawal is somewhat further enhanced. These results suggest that these two proteins have distinct roles that may be related to the divergent regulation of their expression during myeloid differentiation.

Original languageEnglish (US)
Pages (from-to)983-992
Number of pages10
JournalBlood
Volume87
Issue number3
StatePublished - Feb 1 1996

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Interleukin-3
Cell Survival
Cells
Myeloid Cells
Transfection
Genes
Cell death
Cell Death
Cell Line
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Lin, E. Y., Orlofsky, A., Wang, H. G., Reed, J. C., & Prystowsky, M. B. (1996). A1, a Bcl-2 family member, prolongs cell survival and permits myeloid differentiation. Blood, 87(3), 983-992.
Lin, Elaine Y. ; Orlofsky, Amos ; Wang, Hong Gang ; Reed, John C. ; Prystowsky, Michael B. / A1, a Bcl-2 family member, prolongs cell survival and permits myeloid differentiation. In: Blood. 1996 ; Vol. 87, No. 3. pp. 983-992.
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Lin, EY, Orlofsky, A, Wang, HG, Reed, JC & Prystowsky, MB 1996, 'A1, a Bcl-2 family member, prolongs cell survival and permits myeloid differentiation', Blood, vol. 87, no. 3, pp. 983-992.

A1, a Bcl-2 family member, prolongs cell survival and permits myeloid differentiation. / Lin, Elaine Y.; Orlofsky, Amos; Wang, Hong Gang; Reed, John C.; Prystowsky, Michael B.

In: Blood, Vol. 87, No. 3, 01.02.1996, p. 983-992.

Research output: Contribution to journalArticle

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AB - A1, a bcl-2 family member, has been identified as a hematopoietic- specific, early inducible gene. In this study it is shown that stable transfection of A1 into an interleukin-3 (IL-3)dependent myeloid precursor cell line, 32D cl3, leads to a retardation of IL-3 withdrawal-induced cell death similar to that observed with transfection of bcl-2. However, unlike bcl-2, A1 expression permits the accumulation of differentiated myeloid cells both before and after IL-3 withdrawal. Total cell accumulation, on the other hand, is considerably greater after IL-3 deprivation in the bcl-2 transfectant than in A1-expressing cells. Cells cotransfected with the two genes behave similarly to cells singly transfected with bcl-2, except that viability following IL-3 withdrawal is somewhat further enhanced. These results suggest that these two proteins have distinct roles that may be related to the divergent regulation of their expression during myeloid differentiation.

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Lin EY, Orlofsky A, Wang HG, Reed JC, Prystowsky MB. A1, a Bcl-2 family member, prolongs cell survival and permits myeloid differentiation. Blood. 1996 Feb 1;87(3):983-992.