Ablation of caterpillar labial salivary glands: Technique for determining the role of saliva in insect-plant interactions

Richard O. Musser, Edward Farmer, Michelle Peiffer, Spencer A. Williams, Gary W. Felton

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

There has been an ardent interest in herbivore saliva due to its roles in inducing plant defenses and its impact on herbivore fitness. Two techniques are described that inhibit the secretion of labial saliva from the caterpillar, Helicoverpa zea, during feeding. The methods rely on cauterizing the caterpillar's spinneret, the principal secretory structure of the labial glands, or surgically removing the labial salivary gland. Both methods successfully inhibit secretion of saliva and the principal salivary enzyme glucose oxidase. Caterpillars with inhibited saliva production feed at similar rates as the untreated caterpillars, pupate, and emerge as adults. Glucose oxidase has been suggested to increase the caterpillar's survival through the suppression of inducible anti-herbivore defenses in plants. Tobacco (Nicotiana tabacum) leaves fed on by caterpillars with ablated salivary glands had significantly higher levels of nicotine, an inducible anti-herbivore defense compound of tobacco, than leaves fed upon by caterpillars with intact labial salivary glands. Tomato (Lycopersicon esculentum) leaves fed upon by caterpillars with suppressed salivary secretions showed greatly reduced evidence of hydrogen peroxide formation compared to leaves fed upon by intact caterpillars. These two methods are useful techniques for determining the role that saliva plays in manipulating plant anti-herbivore defenses.

Original languageEnglish (US)
Pages (from-to)981-992
Number of pages12
JournalJournal of Chemical Ecology
Volume32
Issue number5
DOIs
StatePublished - May 1 2006

Fingerprint

plant-insect interaction
plant-insect relations
Glucose Oxidase
saliva
Herbivory
Tobacco
caterpillar
lips
Lip
Ablation
salivary glands
Salivary Glands
Saliva
ablation
Insects
insect larvae
Nicotine
antiherbivore defense
Hydrogen Peroxide
herbivores

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry

Cite this

Musser, Richard O. ; Farmer, Edward ; Peiffer, Michelle ; Williams, Spencer A. ; Felton, Gary W. / Ablation of caterpillar labial salivary glands : Technique for determining the role of saliva in insect-plant interactions. In: Journal of Chemical Ecology. 2006 ; Vol. 32, No. 5. pp. 981-992.
@article{e55c338ef5604c36a3c81c97911b36c5,
title = "Ablation of caterpillar labial salivary glands: Technique for determining the role of saliva in insect-plant interactions",
abstract = "There has been an ardent interest in herbivore saliva due to its roles in inducing plant defenses and its impact on herbivore fitness. Two techniques are described that inhibit the secretion of labial saliva from the caterpillar, Helicoverpa zea, during feeding. The methods rely on cauterizing the caterpillar's spinneret, the principal secretory structure of the labial glands, or surgically removing the labial salivary gland. Both methods successfully inhibit secretion of saliva and the principal salivary enzyme glucose oxidase. Caterpillars with inhibited saliva production feed at similar rates as the untreated caterpillars, pupate, and emerge as adults. Glucose oxidase has been suggested to increase the caterpillar's survival through the suppression of inducible anti-herbivore defenses in plants. Tobacco (Nicotiana tabacum) leaves fed on by caterpillars with ablated salivary glands had significantly higher levels of nicotine, an inducible anti-herbivore defense compound of tobacco, than leaves fed upon by caterpillars with intact labial salivary glands. Tomato (Lycopersicon esculentum) leaves fed upon by caterpillars with suppressed salivary secretions showed greatly reduced evidence of hydrogen peroxide formation compared to leaves fed upon by intact caterpillars. These two methods are useful techniques for determining the role that saliva plays in manipulating plant anti-herbivore defenses.",
author = "Musser, {Richard O.} and Edward Farmer and Michelle Peiffer and Williams, {Spencer A.} and Felton, {Gary W.}",
year = "2006",
month = "5",
day = "1",
doi = "10.1007/s10886-006-9049-4",
language = "English (US)",
volume = "32",
pages = "981--992",
journal = "Journal of Chemical Ecology",
issn = "0098-0331",
publisher = "Springer New York",
number = "5",

}

Ablation of caterpillar labial salivary glands : Technique for determining the role of saliva in insect-plant interactions. / Musser, Richard O.; Farmer, Edward; Peiffer, Michelle; Williams, Spencer A.; Felton, Gary W.

In: Journal of Chemical Ecology, Vol. 32, No. 5, 01.05.2006, p. 981-992.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Ablation of caterpillar labial salivary glands

T2 - Technique for determining the role of saliva in insect-plant interactions

AU - Musser, Richard O.

AU - Farmer, Edward

AU - Peiffer, Michelle

AU - Williams, Spencer A.

AU - Felton, Gary W.

PY - 2006/5/1

Y1 - 2006/5/1

N2 - There has been an ardent interest in herbivore saliva due to its roles in inducing plant defenses and its impact on herbivore fitness. Two techniques are described that inhibit the secretion of labial saliva from the caterpillar, Helicoverpa zea, during feeding. The methods rely on cauterizing the caterpillar's spinneret, the principal secretory structure of the labial glands, or surgically removing the labial salivary gland. Both methods successfully inhibit secretion of saliva and the principal salivary enzyme glucose oxidase. Caterpillars with inhibited saliva production feed at similar rates as the untreated caterpillars, pupate, and emerge as adults. Glucose oxidase has been suggested to increase the caterpillar's survival through the suppression of inducible anti-herbivore defenses in plants. Tobacco (Nicotiana tabacum) leaves fed on by caterpillars with ablated salivary glands had significantly higher levels of nicotine, an inducible anti-herbivore defense compound of tobacco, than leaves fed upon by caterpillars with intact labial salivary glands. Tomato (Lycopersicon esculentum) leaves fed upon by caterpillars with suppressed salivary secretions showed greatly reduced evidence of hydrogen peroxide formation compared to leaves fed upon by intact caterpillars. These two methods are useful techniques for determining the role that saliva plays in manipulating plant anti-herbivore defenses.

AB - There has been an ardent interest in herbivore saliva due to its roles in inducing plant defenses and its impact on herbivore fitness. Two techniques are described that inhibit the secretion of labial saliva from the caterpillar, Helicoverpa zea, during feeding. The methods rely on cauterizing the caterpillar's spinneret, the principal secretory structure of the labial glands, or surgically removing the labial salivary gland. Both methods successfully inhibit secretion of saliva and the principal salivary enzyme glucose oxidase. Caterpillars with inhibited saliva production feed at similar rates as the untreated caterpillars, pupate, and emerge as adults. Glucose oxidase has been suggested to increase the caterpillar's survival through the suppression of inducible anti-herbivore defenses in plants. Tobacco (Nicotiana tabacum) leaves fed on by caterpillars with ablated salivary glands had significantly higher levels of nicotine, an inducible anti-herbivore defense compound of tobacco, than leaves fed upon by caterpillars with intact labial salivary glands. Tomato (Lycopersicon esculentum) leaves fed upon by caterpillars with suppressed salivary secretions showed greatly reduced evidence of hydrogen peroxide formation compared to leaves fed upon by intact caterpillars. These two methods are useful techniques for determining the role that saliva plays in manipulating plant anti-herbivore defenses.

UR - http://www.scopus.com/inward/record.url?scp=33744757408&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744757408&partnerID=8YFLogxK

U2 - 10.1007/s10886-006-9049-4

DO - 10.1007/s10886-006-9049-4

M3 - Article

C2 - 16739018

AN - SCOPUS:33744757408

VL - 32

SP - 981

EP - 992

JO - Journal of Chemical Ecology

JF - Journal of Chemical Ecology

SN - 0098-0331

IS - 5

ER -