Abrogation of heparan sulface synthesis in Drosophila disrupts the Wingless, Hedgehog and Decapentaplegic signaling pathways

Douglas J. Bornemann, Jason E. Duncan, William Staatz, Scott Selleck, Rahul Warrior

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

Studies in Drosophila and vertebrate systems have demonstrated that heparan sulfate proteoglycans (HSPGs) play crucial roles in modulating growth factor signaling. We have isolated mutations in sister of tout velu (sotv), a gene that encodes a co-polymerase that synthesizes HSPG glycosaminoglycan (GAG) chains. Our phenotypic and biochemical analyses reveal that HS levels are dramatically reduced in the absence of Sotv or its partner co-polymerase Tout velu (Ttv), suggesting that both copolymerases are essential for GAG synthesis. Furthermore, we find that mutations in sotv and ttv impair Hh, Wg and Decapentaplegic (Dpp) signaling. This contrasts with previous studies that suggested loss of ttv compromises only Hh signaling. Our results may contribute to understanding the biological basis of hereditary multiple exostoses (HME), a disease associated with bone overgrowth that results from mutations in EXT1 and EXT2, the human orthologs of ttv and sotv.

Original languageEnglish (US)
Pages (from-to)1927-1938
Number of pages12
JournalDevelopment
Volume131
Issue number9
DOIs
StatePublished - May 1 2004

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Hedgehogs
Drosophila
Heparan Sulfate Proteoglycans
Glycosaminoglycans
Mutation
Multiple Hereditary Exostoses
Vertebrates
Intercellular Signaling Peptides and Proteins
Bone and Bones
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology

Cite this

Bornemann, Douglas J. ; Duncan, Jason E. ; Staatz, William ; Selleck, Scott ; Warrior, Rahul. / Abrogation of heparan sulface synthesis in Drosophila disrupts the Wingless, Hedgehog and Decapentaplegic signaling pathways. In: Development. 2004 ; Vol. 131, No. 9. pp. 1927-1938.
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Abrogation of heparan sulface synthesis in Drosophila disrupts the Wingless, Hedgehog and Decapentaplegic signaling pathways. / Bornemann, Douglas J.; Duncan, Jason E.; Staatz, William; Selleck, Scott; Warrior, Rahul.

In: Development, Vol. 131, No. 9, 01.05.2004, p. 1927-1938.

Research output: Contribution to journalArticle

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