Spectrin is a major cytoskeletal component of the brain. At least 3 distinct spectrin subtypes are found in mammalian brain: brain spectrin( 240 235) which is confined mainly to axons, brain spectrin( 240 235E) which is localized largely in neural cell bodies and dendrites, and brain spectrin( 240 235A) which is associated only with astrocytes. Recently, Ivy et al. (5) reported that brain spectrin ( 240 235) was located in dendrites when tissues were fixed with 4% paraformaldehyde. To evaluate this matter further, rat cerebellar cortex prepared with and without aldehydes was stained with antibodies to brain spectrin( 240 235) and examined using peroxidase (4-chloro-1-naphthol or avidin-biotin) or rhodamine to visualize the primary antibody. The preparations (10 μm and 40 μm sections) showed that brain spectrin( 240 235) resided largely in axons with occasional staining of neuronal soma (Purkinje cells), but was not observed in dendrites. These results confirm earlier reports [e.g., (10,12)] showing the discrete compartmentalization of brain spectrin( 240 235) in axons and cell bodies. Brain Spectrin Fodrin Cytoskeleton Neuroanatomy Immunocytochemistry.
All Science Journal Classification (ASJC) codes