Accurate thermometry based on the red and green fluorescence intensity ratio in NaYF4: Yb, Er nanocrystals for bioapplication

Lixin Liu, Feng Qin, Tianquan Lv, Zhiguo Zhang, Wenwu Cao

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Abstract

A biological temperature measurement method based on the fluorescence intensity ratio (FIR) was developed to reduce uncertainty. The upconversion luminescence of NaYF4:Yb, Er nanocrystals was studied as a function of temperature around the physiologically relevant range of 300-330 K. We found that the green-green FIR Fe and red-green FIR (I660/I540) varied linearly as temperature increased. The thermometric uncertainties using the two FIRs were discussed and were determined to be almost constant at 0.6 and 0.09 K for green-green and red-green, respectively. The lower thermometric uncertainty comes from the intense signal-to-noise ratio of the measured FIRs owing to their comparable fluorescence intensities.

Original languageEnglish (US)
Pages (from-to)4664-4667
Number of pages4
JournalOptics Letters
Volume41
Issue number20
DOIs
StatePublished - Oct 15 2016

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All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

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