Acetylated hsp70 and KAP1-mediated Vps34 SUMOylation is required for autophagosome creation in autophagy

Yonghua Yang, Warren Fiskus, Bao Yong, Peter Atadja, Yoshinori Takahashi, Tej K. Pandita, Hong Gang Wang, Kapil N. Bhalla

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Autophagy is a stress-induced catabolic process in which cytoplasmic components, sequestered in double-membrane autophagic vesicles (AVs) or autophagosomes, are delivered to lysosomes for degradation and recycling [Kroemer G, Mariño G, Levine B (2010) Mol Cell 40(2):280-293]. Activity of the class III phosphatidylinositol-3-OH-kinase (PI3K) vacuolar protein-sorting (Vps) 34, bound to coiled-coil moesin-like B-cell lymphoma 2 (Bcl-2)-interacting protein Beclin-1, is required for phosphoinositide generation, essential for AV formation in autophagy [Cuervo AM (2010) Nat Cell Biol 12(8):735-737]. However, how autophagy-inducing stress regulates Vps34 activity has not beenfully elucidated. Ourfindings demonstrate that autoph-agy-inducing stress increases intracellular levels of acetylated induc-ible heat shock protein (hsp) 70, which binds to the Beclin-1-Vps34 complex. Acetylated hsp70 also recruits E3 ligase for SUMOylation, KRAB-ZFP-associated protein 1 (KAP1), inducing Lys840 SUMOylation and increasing Vps34 activity bound to Beclin 1. Knockdown of hsp70 abolished the Beclin-1-Vps34 complex formation, as well as inhibited KAP1 binding to Vps34 and AV formation. Notably, autophagy-inducing stress due to histone deacetylase inhibitor treatment induced AV formation in the wild-type but not hsp70.1/3 knockout mouse embryonic fibroblasts MEFs. These findings highlight a regulatory mechanism of Vps34 activity, which involves acetylated hsp70 and KAP1-dependent SUMOylation of Vps34 bound to Beclin 1.

Original languageEnglish (US)
Pages (from-to)6841-6846
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number17
DOIs
StatePublished - Apr 23 2013

Fingerprint

Sumoylation
Autophagy
Proteins
Class III Phosphatidylinositol 3-Kinases
Histone Deacetylase Inhibitors
HSP70 Heat-Shock Proteins
Ubiquitin-Protein Ligases
Recycling
B-Cell Lymphoma
Protein Transport
Phosphatidylinositols
Lysosomes
Protein Binding
Knockout Mice
Fibroblasts
Beclin-1
Autophagosomes
Membranes

All Science Journal Classification (ASJC) codes

  • General

Cite this

@article{230934ffe5ad4dfa97bb69fb632eedb3,
title = "Acetylated hsp70 and KAP1-mediated Vps34 SUMOylation is required for autophagosome creation in autophagy",
abstract = "Autophagy is a stress-induced catabolic process in which cytoplasmic components, sequestered in double-membrane autophagic vesicles (AVs) or autophagosomes, are delivered to lysosomes for degradation and recycling [Kroemer G, Mari{\~n}o G, Levine B (2010) Mol Cell 40(2):280-293]. Activity of the class III phosphatidylinositol-3-OH-kinase (PI3K) vacuolar protein-sorting (Vps) 34, bound to coiled-coil moesin-like B-cell lymphoma 2 (Bcl-2)-interacting protein Beclin-1, is required for phosphoinositide generation, essential for AV formation in autophagy [Cuervo AM (2010) Nat Cell Biol 12(8):735-737]. However, how autophagy-inducing stress regulates Vps34 activity has not beenfully elucidated. Ourfindings demonstrate that autoph-agy-inducing stress increases intracellular levels of acetylated induc-ible heat shock protein (hsp) 70, which binds to the Beclin-1-Vps34 complex. Acetylated hsp70 also recruits E3 ligase for SUMOylation, KRAB-ZFP-associated protein 1 (KAP1), inducing Lys840 SUMOylation and increasing Vps34 activity bound to Beclin 1. Knockdown of hsp70 abolished the Beclin-1-Vps34 complex formation, as well as inhibited KAP1 binding to Vps34 and AV formation. Notably, autophagy-inducing stress due to histone deacetylase inhibitor treatment induced AV formation in the wild-type but not hsp70.1/3 knockout mouse embryonic fibroblasts MEFs. These findings highlight a regulatory mechanism of Vps34 activity, which involves acetylated hsp70 and KAP1-dependent SUMOylation of Vps34 bound to Beclin 1.",
author = "Yonghua Yang and Warren Fiskus and Bao Yong and Peter Atadja and Yoshinori Takahashi and Pandita, {Tej K.} and Wang, {Hong Gang} and Bhalla, {Kapil N.}",
year = "2013",
month = "4",
day = "23",
doi = "10.1073/pnas.1217692110",
language = "English (US)",
volume = "110",
pages = "6841--6846",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "17",

}

Acetylated hsp70 and KAP1-mediated Vps34 SUMOylation is required for autophagosome creation in autophagy. / Yang, Yonghua; Fiskus, Warren; Yong, Bao; Atadja, Peter; Takahashi, Yoshinori; Pandita, Tej K.; Wang, Hong Gang; Bhalla, Kapil N.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 17, 23.04.2013, p. 6841-6846.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Acetylated hsp70 and KAP1-mediated Vps34 SUMOylation is required for autophagosome creation in autophagy

AU - Yang, Yonghua

AU - Fiskus, Warren

AU - Yong, Bao

AU - Atadja, Peter

AU - Takahashi, Yoshinori

AU - Pandita, Tej K.

AU - Wang, Hong Gang

AU - Bhalla, Kapil N.

PY - 2013/4/23

Y1 - 2013/4/23

N2 - Autophagy is a stress-induced catabolic process in which cytoplasmic components, sequestered in double-membrane autophagic vesicles (AVs) or autophagosomes, are delivered to lysosomes for degradation and recycling [Kroemer G, Mariño G, Levine B (2010) Mol Cell 40(2):280-293]. Activity of the class III phosphatidylinositol-3-OH-kinase (PI3K) vacuolar protein-sorting (Vps) 34, bound to coiled-coil moesin-like B-cell lymphoma 2 (Bcl-2)-interacting protein Beclin-1, is required for phosphoinositide generation, essential for AV formation in autophagy [Cuervo AM (2010) Nat Cell Biol 12(8):735-737]. However, how autophagy-inducing stress regulates Vps34 activity has not beenfully elucidated. Ourfindings demonstrate that autoph-agy-inducing stress increases intracellular levels of acetylated induc-ible heat shock protein (hsp) 70, which binds to the Beclin-1-Vps34 complex. Acetylated hsp70 also recruits E3 ligase for SUMOylation, KRAB-ZFP-associated protein 1 (KAP1), inducing Lys840 SUMOylation and increasing Vps34 activity bound to Beclin 1. Knockdown of hsp70 abolished the Beclin-1-Vps34 complex formation, as well as inhibited KAP1 binding to Vps34 and AV formation. Notably, autophagy-inducing stress due to histone deacetylase inhibitor treatment induced AV formation in the wild-type but not hsp70.1/3 knockout mouse embryonic fibroblasts MEFs. These findings highlight a regulatory mechanism of Vps34 activity, which involves acetylated hsp70 and KAP1-dependent SUMOylation of Vps34 bound to Beclin 1.

AB - Autophagy is a stress-induced catabolic process in which cytoplasmic components, sequestered in double-membrane autophagic vesicles (AVs) or autophagosomes, are delivered to lysosomes for degradation and recycling [Kroemer G, Mariño G, Levine B (2010) Mol Cell 40(2):280-293]. Activity of the class III phosphatidylinositol-3-OH-kinase (PI3K) vacuolar protein-sorting (Vps) 34, bound to coiled-coil moesin-like B-cell lymphoma 2 (Bcl-2)-interacting protein Beclin-1, is required for phosphoinositide generation, essential for AV formation in autophagy [Cuervo AM (2010) Nat Cell Biol 12(8):735-737]. However, how autophagy-inducing stress regulates Vps34 activity has not beenfully elucidated. Ourfindings demonstrate that autoph-agy-inducing stress increases intracellular levels of acetylated induc-ible heat shock protein (hsp) 70, which binds to the Beclin-1-Vps34 complex. Acetylated hsp70 also recruits E3 ligase for SUMOylation, KRAB-ZFP-associated protein 1 (KAP1), inducing Lys840 SUMOylation and increasing Vps34 activity bound to Beclin 1. Knockdown of hsp70 abolished the Beclin-1-Vps34 complex formation, as well as inhibited KAP1 binding to Vps34 and AV formation. Notably, autophagy-inducing stress due to histone deacetylase inhibitor treatment induced AV formation in the wild-type but not hsp70.1/3 knockout mouse embryonic fibroblasts MEFs. These findings highlight a regulatory mechanism of Vps34 activity, which involves acetylated hsp70 and KAP1-dependent SUMOylation of Vps34 bound to Beclin 1.

UR - http://www.scopus.com/inward/record.url?scp=84876865718&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876865718&partnerID=8YFLogxK

U2 - 10.1073/pnas.1217692110

DO - 10.1073/pnas.1217692110

M3 - Article

C2 - 23569248

AN - SCOPUS:84876865718

VL - 110

SP - 6841

EP - 6846

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 17

ER -