Acid-growth response and α-expansins in suspension cultures of bright yellow 2 tobacco

Bruce M. Link, Daniel J. Cosgrove

Research output: Contribution to journalArticle

97 Scopus citations

Abstract

The possibility that Bright Yellow 2 (BY2) tobacco (Nicotiana tabacum L.) suspension-cultured cells possess an expansin-mediated acid-growth mechanism was examined by multiple approaches. BY2 cells grew three times faster upon treatment with fusicoccin, which induces an acidification of the cell wall. Exogenous expansins like-wise stimulated BY2 cell growth 3-fold. Protein extracted from BY2 cell walls possessed the expansin-like ability to induce extension of isolated walls. In western-blot analysis of BY2 wall protein, one band of 29 kD was recognized by anti-expansin antibody. Six different classes of α-expansin mRNA were identified in a BY2 cDNA library. Northern-blot analysis indicated moderate to low abundance of multiple α-expansin mRNAs in BY2 cells. From these results we conclude that BY2 suspension-cultured cells have the necessary components for expansin-mediated cell wall enlargement.

Original languageEnglish (US)
Pages (from-to)907-916
Number of pages10
JournalPlant physiology
Volume118
Issue number3
DOIs
StatePublished - Jan 1 1998

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

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