Acoustofluidic chemical waveform generator and switch

Daniel Ahmed, Hari S. Muddana, Mengqian Lu, Jarrod B. French, Adem Ozcelik, Ye Fang, Peter J. Butler, Stephen J. Benkovic, Andreas Manz, Tony Jun Huang

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Eliciting a cellular response to a changing chemical microenvironment is central to many biological processes including gene expression, cell migration, differentiation, apoptosis, and intercellular signaling. The nature and scope of the response is highly dependent upon the spatiotemporal characteristics of the stimulus. To date, studies that investigate this phenomenon have been limited to digital (or step) chemical stimulation with little control over the temporal counterparts. Here, we demonstrate an acoustofluidic (i.e., fusion of acoustics and microfluidics) approach for generating programmable chemical waveforms that permits continuous modulation of the signal characteristics including the amplitude (i.e., sample concentration), shape, frequency, and duty cycle, with frequencies reaching up to 30 Hz. Furthermore, we show fast switching between multiple distinct stimuli, wherein the waveform of each stimulus is independently controlled. Using our device, we characterized the frequency-dependent activation and internalization of the β2-adrenergic receptor (β2-AR), a prototypic G-protein coupled receptor (GPCR), using epinephrine. The acoustofluidic-based programmable chemical waveform generation and switching method presented herein is expected to be a powerful tool for the investigation and characterization of the kinetics and other dynamic properties of many biological and biochemical processes.

Original languageEnglish (US)
Pages (from-to)11803-11810
Number of pages8
JournalAnalytical chemistry
Volume86
Issue number23
DOIs
StatePublished - Dec 2 2014

Fingerprint

Switches
Microfluidics
Fusion reactions
Acoustics
G-Protein-Coupled Receptors
Gene expression
Adrenergic Receptors
Epinephrine
Chemical activation
Modulation
Apoptosis
Kinetics

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Ahmed, D., Muddana, H. S., Lu, M., French, J. B., Ozcelik, A., Fang, Y., ... Huang, T. J. (2014). Acoustofluidic chemical waveform generator and switch. Analytical chemistry, 86(23), 11803-11810. https://doi.org/10.1021/ac5033676
Ahmed, Daniel ; Muddana, Hari S. ; Lu, Mengqian ; French, Jarrod B. ; Ozcelik, Adem ; Fang, Ye ; Butler, Peter J. ; Benkovic, Stephen J. ; Manz, Andreas ; Huang, Tony Jun. / Acoustofluidic chemical waveform generator and switch. In: Analytical chemistry. 2014 ; Vol. 86, No. 23. pp. 11803-11810.
@article{5ff4475965214df7b004c1da5a489f0c,
title = "Acoustofluidic chemical waveform generator and switch",
abstract = "Eliciting a cellular response to a changing chemical microenvironment is central to many biological processes including gene expression, cell migration, differentiation, apoptosis, and intercellular signaling. The nature and scope of the response is highly dependent upon the spatiotemporal characteristics of the stimulus. To date, studies that investigate this phenomenon have been limited to digital (or step) chemical stimulation with little control over the temporal counterparts. Here, we demonstrate an acoustofluidic (i.e., fusion of acoustics and microfluidics) approach for generating programmable chemical waveforms that permits continuous modulation of the signal characteristics including the amplitude (i.e., sample concentration), shape, frequency, and duty cycle, with frequencies reaching up to 30 Hz. Furthermore, we show fast switching between multiple distinct stimuli, wherein the waveform of each stimulus is independently controlled. Using our device, we characterized the frequency-dependent activation and internalization of the β2-adrenergic receptor (β2-AR), a prototypic G-protein coupled receptor (GPCR), using epinephrine. The acoustofluidic-based programmable chemical waveform generation and switching method presented herein is expected to be a powerful tool for the investigation and characterization of the kinetics and other dynamic properties of many biological and biochemical processes.",
author = "Daniel Ahmed and Muddana, {Hari S.} and Mengqian Lu and French, {Jarrod B.} and Adem Ozcelik and Ye Fang and Butler, {Peter J.} and Benkovic, {Stephen J.} and Andreas Manz and Huang, {Tony Jun}",
year = "2014",
month = "12",
day = "2",
doi = "10.1021/ac5033676",
language = "English (US)",
volume = "86",
pages = "11803--11810",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "23",

}

Ahmed, D, Muddana, HS, Lu, M, French, JB, Ozcelik, A, Fang, Y, Butler, PJ, Benkovic, SJ, Manz, A & Huang, TJ 2014, 'Acoustofluidic chemical waveform generator and switch', Analytical chemistry, vol. 86, no. 23, pp. 11803-11810. https://doi.org/10.1021/ac5033676

Acoustofluidic chemical waveform generator and switch. / Ahmed, Daniel; Muddana, Hari S.; Lu, Mengqian; French, Jarrod B.; Ozcelik, Adem; Fang, Ye; Butler, Peter J.; Benkovic, Stephen J.; Manz, Andreas; Huang, Tony Jun.

In: Analytical chemistry, Vol. 86, No. 23, 02.12.2014, p. 11803-11810.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Acoustofluidic chemical waveform generator and switch

AU - Ahmed, Daniel

AU - Muddana, Hari S.

AU - Lu, Mengqian

AU - French, Jarrod B.

AU - Ozcelik, Adem

AU - Fang, Ye

AU - Butler, Peter J.

AU - Benkovic, Stephen J.

AU - Manz, Andreas

AU - Huang, Tony Jun

PY - 2014/12/2

Y1 - 2014/12/2

N2 - Eliciting a cellular response to a changing chemical microenvironment is central to many biological processes including gene expression, cell migration, differentiation, apoptosis, and intercellular signaling. The nature and scope of the response is highly dependent upon the spatiotemporal characteristics of the stimulus. To date, studies that investigate this phenomenon have been limited to digital (or step) chemical stimulation with little control over the temporal counterparts. Here, we demonstrate an acoustofluidic (i.e., fusion of acoustics and microfluidics) approach for generating programmable chemical waveforms that permits continuous modulation of the signal characteristics including the amplitude (i.e., sample concentration), shape, frequency, and duty cycle, with frequencies reaching up to 30 Hz. Furthermore, we show fast switching between multiple distinct stimuli, wherein the waveform of each stimulus is independently controlled. Using our device, we characterized the frequency-dependent activation and internalization of the β2-adrenergic receptor (β2-AR), a prototypic G-protein coupled receptor (GPCR), using epinephrine. The acoustofluidic-based programmable chemical waveform generation and switching method presented herein is expected to be a powerful tool for the investigation and characterization of the kinetics and other dynamic properties of many biological and biochemical processes.

AB - Eliciting a cellular response to a changing chemical microenvironment is central to many biological processes including gene expression, cell migration, differentiation, apoptosis, and intercellular signaling. The nature and scope of the response is highly dependent upon the spatiotemporal characteristics of the stimulus. To date, studies that investigate this phenomenon have been limited to digital (or step) chemical stimulation with little control over the temporal counterparts. Here, we demonstrate an acoustofluidic (i.e., fusion of acoustics and microfluidics) approach for generating programmable chemical waveforms that permits continuous modulation of the signal characteristics including the amplitude (i.e., sample concentration), shape, frequency, and duty cycle, with frequencies reaching up to 30 Hz. Furthermore, we show fast switching between multiple distinct stimuli, wherein the waveform of each stimulus is independently controlled. Using our device, we characterized the frequency-dependent activation and internalization of the β2-adrenergic receptor (β2-AR), a prototypic G-protein coupled receptor (GPCR), using epinephrine. The acoustofluidic-based programmable chemical waveform generation and switching method presented herein is expected to be a powerful tool for the investigation and characterization of the kinetics and other dynamic properties of many biological and biochemical processes.

UR - http://www.scopus.com/inward/record.url?scp=84915820010&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84915820010&partnerID=8YFLogxK

U2 - 10.1021/ac5033676

DO - 10.1021/ac5033676

M3 - Article

C2 - 25405550

AN - SCOPUS:84915820010

VL - 86

SP - 11803

EP - 11810

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 23

ER -

Ahmed D, Muddana HS, Lu M, French JB, Ozcelik A, Fang Y et al. Acoustofluidic chemical waveform generator and switch. Analytical chemistry. 2014 Dec 2;86(23):11803-11810. https://doi.org/10.1021/ac5033676