Acoustofluidic Transfer of Inflammatory Cells from Human Sputum Samples

Sixing Li, Liqiang Ren, Po Hsun Huang, Xianglan Yao, Rosemarie A. Cuento, J. Philip McCoy, Craig Eugene Cameron, Stewart J. Levine, Tony Jun Huang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

For sputum analysis, the transfer of inflammatory cells from liquefied sputum samples to a culture medium or buffer solution is a critical step because it removes the inflammatory cells from the presence of residual dithiothreitol (DTT), a reagent that reduces cell viability and interferes with further sputum analyses. In this work, we report an acoustofluidic platform for transferring inflammatory cells using standing surface acoustic waves (SSAW). In particular, we exploit the acoustic radiation force generated from a SSAW field to actively transfer inflammatory cells from a solution containing residual DTT to a buffer solution. The viability and integrity of the inflammatory cells are maintained during the acoustofluidic-based cell transfer process. Our acoustofluidic technique removes residual DTT generated in sputum liquefaction and facilitates immunophenotyping of major inflammatory cells from sputum samples. It enables cell transfer in a continuous flow, which AIDS the development of an automated, integrated system for on-chip sputum processing and analysis.

Original languageEnglish (US)
Pages (from-to)5655-5661
Number of pages7
JournalAnalytical Chemistry
Volume88
Issue number11
DOIs
StatePublished - Jun 7 2016

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Dithiothreitol
Surface waves
Buffers
Acoustic waves
Liquefaction
Culture Media
Acoustics
Cells
Radiation
Processing

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Li, S., Ren, L., Huang, P. H., Yao, X., Cuento, R. A., McCoy, J. P., ... Huang, T. J. (2016). Acoustofluidic Transfer of Inflammatory Cells from Human Sputum Samples. Analytical Chemistry, 88(11), 5655-5661. https://doi.org/10.1021/acs.analchem.5b03383
Li, Sixing ; Ren, Liqiang ; Huang, Po Hsun ; Yao, Xianglan ; Cuento, Rosemarie A. ; McCoy, J. Philip ; Cameron, Craig Eugene ; Levine, Stewart J. ; Huang, Tony Jun. / Acoustofluidic Transfer of Inflammatory Cells from Human Sputum Samples. In: Analytical Chemistry. 2016 ; Vol. 88, No. 11. pp. 5655-5661.
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Li, S, Ren, L, Huang, PH, Yao, X, Cuento, RA, McCoy, JP, Cameron, CE, Levine, SJ & Huang, TJ 2016, 'Acoustofluidic Transfer of Inflammatory Cells from Human Sputum Samples', Analytical Chemistry, vol. 88, no. 11, pp. 5655-5661. https://doi.org/10.1021/acs.analchem.5b03383

Acoustofluidic Transfer of Inflammatory Cells from Human Sputum Samples. / Li, Sixing; Ren, Liqiang; Huang, Po Hsun; Yao, Xianglan; Cuento, Rosemarie A.; McCoy, J. Philip; Cameron, Craig Eugene; Levine, Stewart J.; Huang, Tony Jun.

In: Analytical Chemistry, Vol. 88, No. 11, 07.06.2016, p. 5655-5661.

Research output: Contribution to journalArticle

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AU - Huang, Tony Jun

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Li S, Ren L, Huang PH, Yao X, Cuento RA, McCoy JP et al. Acoustofluidic Transfer of Inflammatory Cells from Human Sputum Samples. Analytical Chemistry. 2016 Jun 7;88(11):5655-5661. https://doi.org/10.1021/acs.analchem.5b03383