TY - JOUR
T1 - Acquired antibody responses against merozoite surface protein-119 antigen during Plasmodium falciparum and P.vivax infections in South Indian city of Mangaluru
AU - Punnath, Kishore
AU - Dayanand, Kiran K.
AU - Midya, Vishal
AU - Chandrashekar, Valleesha N.
AU - Achur, Rajeshwara N.
AU - Kakkilaya, Srinivas B.
AU - Ghosh, Susanta K.
AU - Kumari, Suchetha N.
AU - Gowda, D. Channe
N1 - Funding Information:
The authors thank the study participants for their consent to participate in the study. We thank MR4 for providing us the recombinant antigens used in ELISA; MRA 53-Plasmodium falciparum yP30P2 PfMSP-119 (Q-NG)FVO/VK1, deposited by DC Kaslow, obtained through the MR4 and MRA 47-Plasmodium vivax yPv 200 MSP119 2905/6 deposited by DC Kaslow as part of the BEI Resources Repository, NIAID, NIH Manassas, VA. We thank Dr. Rajeshwari Devi, District medical officer and superintendent of District Wenlock Hospital for her support and guidance, Dr. Arun Kumar, District Vector Borne Disease Control Programme officer, Dakshina Kannada, for his support and the Mangalore City Corporation Health officials for their kind help to conduct the study. This work was supported by the Grant D43 TW008268 from the Fogarty International Center of the National Institutes of Health, USA, to DCG, under the Global Infectious Diseases Program.
Funding Information:
This work was supported by the Grant D43 TW008268 from the Fogarty International Center of the National Institutes of Health, USA, to DCG under the Global Infectious Diseases Program. The funders had no role in study design, data collection, and analysis, decision to publish, or preparation of the manuscript. Acknowledgements
Publisher Copyright:
© 2020, Indian Society for Parasitology.
PY - 2021/3
Y1 - 2021/3
N2 - Merozoite surface protein-1 (MSP-1) of malaria parasites has been extensively studied as a malaria vaccine candidate and the antibody response to this protein is an important indicator of protective immunity to malaria. Mangaluru city and its surrounding areas in southwestern India are endemic to malaria with Plasmodium vivax being the most widespread and prevalent species although P. falciparum also frequently infects. However, no information is available on the level of protective immunity in this population. In this regard, a prospective hospital-based study was performed in malarial patients to assess antibody responses against the 19-kDa C-terminal portion of P. vivax and P. falciparum MSP-1 (MSP-119). Serum samples from 51 healthy endemic controls and 267 infected individuals were collected and anti-MSP-119 antibody levels were analyzed by ELISA. The possible association between the antibody responses and morbidity parameters such as malarial anemia and thrombocytopenia was investigated. Among the 267 infected cases, 144 had P. vivax and 123 had P. falciparum infections. Significant levels of anti-MSP-119 antibody were observed both in P. vivax (123/144; 85.4%) and P. falciparum (108/123; 87.9%) infected individuals. In both type of infections, the major antibody isotypes were IgG1 and IgG3. The IgG levels were found to be increased in patients with severe anemia and thrombocytopenia. The antibody levels were also higher in infected individuals who had several previous infections, although antibodies produced during previous infections were short lived. The predominance of cytophilic anti-MSP-119 IgG1 and IgG3 antibodies suggests the possibility of a dual role of Pv MSP-119 and Pf MSP-119 during malarial immunity and pathogenesis.
AB - Merozoite surface protein-1 (MSP-1) of malaria parasites has been extensively studied as a malaria vaccine candidate and the antibody response to this protein is an important indicator of protective immunity to malaria. Mangaluru city and its surrounding areas in southwestern India are endemic to malaria with Plasmodium vivax being the most widespread and prevalent species although P. falciparum also frequently infects. However, no information is available on the level of protective immunity in this population. In this regard, a prospective hospital-based study was performed in malarial patients to assess antibody responses against the 19-kDa C-terminal portion of P. vivax and P. falciparum MSP-1 (MSP-119). Serum samples from 51 healthy endemic controls and 267 infected individuals were collected and anti-MSP-119 antibody levels were analyzed by ELISA. The possible association between the antibody responses and morbidity parameters such as malarial anemia and thrombocytopenia was investigated. Among the 267 infected cases, 144 had P. vivax and 123 had P. falciparum infections. Significant levels of anti-MSP-119 antibody were observed both in P. vivax (123/144; 85.4%) and P. falciparum (108/123; 87.9%) infected individuals. In both type of infections, the major antibody isotypes were IgG1 and IgG3. The IgG levels were found to be increased in patients with severe anemia and thrombocytopenia. The antibody levels were also higher in infected individuals who had several previous infections, although antibodies produced during previous infections were short lived. The predominance of cytophilic anti-MSP-119 IgG1 and IgG3 antibodies suggests the possibility of a dual role of Pv MSP-119 and Pf MSP-119 during malarial immunity and pathogenesis.
UR - http://www.scopus.com/inward/record.url?scp=85093067825&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85093067825&partnerID=8YFLogxK
U2 - 10.1007/s12639-020-01288-4
DO - 10.1007/s12639-020-01288-4
M3 - Article
C2 - 33100734
AN - SCOPUS:85093067825
VL - 45
SP - 176
EP - 190
JO - Journal of Parasitic Diseases
JF - Journal of Parasitic Diseases
SN - 0971-7196
IS - 1
ER -
