Acquisition of inorganic carbon by the marine diatom Thalassiosira weissflogii

François M.M. Morel, Elizabeth H. Cox, Anne M.L. Kraepiel, Todd W. Lane, Allen J. Milligan, Irene Schaperdoth, John R. Reinfelder, Philippe D. Tortell

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Recent data on the physiology of inorganic carbon acquisition by the model marine diatom Thalassiosira weissflogii (Grunow) demonstrate the importance of the catalytic equilibration of HCO3- and CO2 by carbonic anhydrases located in the periplasm and in the cytoplasm. These enzymes can use Zn, Co or Cd as their metal centre, and their activity increases at low ambient CO2. The silica frustule provides buffering for extracellular CA activity. The transmembrane transport of CO2 may occur by passive diffusion. Under CO2 limitation, the cytoplasmic HCO3- is used to form malate and oxaloacetic acid via phosphoenolpyruvate carboxylase. It appears that subsequent decarboxylation of these compounds in the chloroplast regenerates CO2 near the site of Rubisco, and thus provides the organism with an effective unicellular C4 photosynthetic pathway. These results, together with other published data, bring up two major questions regarding inorganic carbon acquisition in diatoms: What is the major species of inorganic carbon (CO2 or HCO3-) transported across the membrane under natural conditions? And what is the form of carbon (inorganic or organic) accumulated by the cells?

Original languageEnglish (US)
Pages (from-to)301-308
Number of pages8
JournalFunctional Plant Biology
Volume29
Issue number2-3
StatePublished - Aug 19 2002

Fingerprint

Bacillariophyceae
Thalassiosira
carbon dioxide
carbon
oxaloacetic acid
decarboxylation
phosphoenolpyruvate carboxylase
carbonate dehydratase
ribulose-bisphosphate carboxylase
malates
silica
cytoplasm
physiology
chloroplasts
metals
organisms
enzymes
cells

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Morel, F. M. M., Cox, E. H., Kraepiel, A. M. L., Lane, T. W., Milligan, A. J., Schaperdoth, I., ... Tortell, P. D. (2002). Acquisition of inorganic carbon by the marine diatom Thalassiosira weissflogii. Functional Plant Biology, 29(2-3), 301-308.
Morel, François M.M. ; Cox, Elizabeth H. ; Kraepiel, Anne M.L. ; Lane, Todd W. ; Milligan, Allen J. ; Schaperdoth, Irene ; Reinfelder, John R. ; Tortell, Philippe D. / Acquisition of inorganic carbon by the marine diatom Thalassiosira weissflogii. In: Functional Plant Biology. 2002 ; Vol. 29, No. 2-3. pp. 301-308.
@article{b7f7a0b8911448a3a74a7fb79a94d6eb,
title = "Acquisition of inorganic carbon by the marine diatom Thalassiosira weissflogii",
abstract = "Recent data on the physiology of inorganic carbon acquisition by the model marine diatom Thalassiosira weissflogii (Grunow) demonstrate the importance of the catalytic equilibration of HCO3- and CO2 by carbonic anhydrases located in the periplasm and in the cytoplasm. These enzymes can use Zn, Co or Cd as their metal centre, and their activity increases at low ambient CO2. The silica frustule provides buffering for extracellular CA activity. The transmembrane transport of CO2 may occur by passive diffusion. Under CO2 limitation, the cytoplasmic HCO3- is used to form malate and oxaloacetic acid via phosphoenolpyruvate carboxylase. It appears that subsequent decarboxylation of these compounds in the chloroplast regenerates CO2 near the site of Rubisco, and thus provides the organism with an effective unicellular C4 photosynthetic pathway. These results, together with other published data, bring up two major questions regarding inorganic carbon acquisition in diatoms: What is the major species of inorganic carbon (CO2 or HCO3-) transported across the membrane under natural conditions? And what is the form of carbon (inorganic or organic) accumulated by the cells?",
author = "Morel, {Fran{\cc}ois M.M.} and Cox, {Elizabeth H.} and Kraepiel, {Anne M.L.} and Lane, {Todd W.} and Milligan, {Allen J.} and Irene Schaperdoth and Reinfelder, {John R.} and Tortell, {Philippe D.}",
year = "2002",
month = "8",
day = "19",
language = "English (US)",
volume = "29",
pages = "301--308",
journal = "Functional Plant Biology",
issn = "1445-4408",
publisher = "CSIRO",
number = "2-3",

}

Morel, FMM, Cox, EH, Kraepiel, AML, Lane, TW, Milligan, AJ, Schaperdoth, I, Reinfelder, JR & Tortell, PD 2002, 'Acquisition of inorganic carbon by the marine diatom Thalassiosira weissflogii', Functional Plant Biology, vol. 29, no. 2-3, pp. 301-308.

Acquisition of inorganic carbon by the marine diatom Thalassiosira weissflogii. / Morel, François M.M.; Cox, Elizabeth H.; Kraepiel, Anne M.L.; Lane, Todd W.; Milligan, Allen J.; Schaperdoth, Irene; Reinfelder, John R.; Tortell, Philippe D.

In: Functional Plant Biology, Vol. 29, No. 2-3, 19.08.2002, p. 301-308.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Acquisition of inorganic carbon by the marine diatom Thalassiosira weissflogii

AU - Morel, François M.M.

AU - Cox, Elizabeth H.

AU - Kraepiel, Anne M.L.

AU - Lane, Todd W.

AU - Milligan, Allen J.

AU - Schaperdoth, Irene

AU - Reinfelder, John R.

AU - Tortell, Philippe D.

PY - 2002/8/19

Y1 - 2002/8/19

N2 - Recent data on the physiology of inorganic carbon acquisition by the model marine diatom Thalassiosira weissflogii (Grunow) demonstrate the importance of the catalytic equilibration of HCO3- and CO2 by carbonic anhydrases located in the periplasm and in the cytoplasm. These enzymes can use Zn, Co or Cd as their metal centre, and their activity increases at low ambient CO2. The silica frustule provides buffering for extracellular CA activity. The transmembrane transport of CO2 may occur by passive diffusion. Under CO2 limitation, the cytoplasmic HCO3- is used to form malate and oxaloacetic acid via phosphoenolpyruvate carboxylase. It appears that subsequent decarboxylation of these compounds in the chloroplast regenerates CO2 near the site of Rubisco, and thus provides the organism with an effective unicellular C4 photosynthetic pathway. These results, together with other published data, bring up two major questions regarding inorganic carbon acquisition in diatoms: What is the major species of inorganic carbon (CO2 or HCO3-) transported across the membrane under natural conditions? And what is the form of carbon (inorganic or organic) accumulated by the cells?

AB - Recent data on the physiology of inorganic carbon acquisition by the model marine diatom Thalassiosira weissflogii (Grunow) demonstrate the importance of the catalytic equilibration of HCO3- and CO2 by carbonic anhydrases located in the periplasm and in the cytoplasm. These enzymes can use Zn, Co or Cd as their metal centre, and their activity increases at low ambient CO2. The silica frustule provides buffering for extracellular CA activity. The transmembrane transport of CO2 may occur by passive diffusion. Under CO2 limitation, the cytoplasmic HCO3- is used to form malate and oxaloacetic acid via phosphoenolpyruvate carboxylase. It appears that subsequent decarboxylation of these compounds in the chloroplast regenerates CO2 near the site of Rubisco, and thus provides the organism with an effective unicellular C4 photosynthetic pathway. These results, together with other published data, bring up two major questions regarding inorganic carbon acquisition in diatoms: What is the major species of inorganic carbon (CO2 or HCO3-) transported across the membrane under natural conditions? And what is the form of carbon (inorganic or organic) accumulated by the cells?

UR - http://www.scopus.com/inward/record.url?scp=0036330101&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036330101&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0036330101

VL - 29

SP - 301

EP - 308

JO - Functional Plant Biology

JF - Functional Plant Biology

SN - 1445-4408

IS - 2-3

ER -

Morel FMM, Cox EH, Kraepiel AML, Lane TW, Milligan AJ, Schaperdoth I et al. Acquisition of inorganic carbon by the marine diatom Thalassiosira weissflogii. Functional Plant Biology. 2002 Aug 19;29(2-3):301-308.