Acquisition of the metastatic phenotype is accompanied by H 2O2-dependent activation of the p130cas signaling complex

Nadine Hempel, Toni R. Bartling, Badar Mian, J. Andres Melendez

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) have emerged as cellular signaling molecules and are implicated in metastatic disease by their ability to drive invasion and migration. Here, we define the signaling adaptor protein p130Cas (Crkassociated substrate) as a key redox-responsive molecular trigger that is engaged in highly invasive metastatic bladder tumor cell lines. Endogenous shifts in steady-state hydrogen peroxide (H2O2) that accompany the metastatic phenotype increase p130Cas phosphorylation, membrane recruitment and association with the scaffolding protein Crk, and subsequent Rac1 activation and actin reorganization. Both enzymatic and nonenzymatic scavenging of H 2O2 abrogates p130Cas-dependent signaling and the migratory and invasive activity of the metastatic bladder tumor cells. Disruption of p130Cas attenuates both invasion and migration of the metastatic variant (253J-BV). 253J-BV cells displayed an increase in global thiol oxidation and a concomitant decrease in total phosphatase activity, common target proteins of active-site cysteine oxidation. The dependence of phosphatases on regulation of p130Cas was highlighted when depletion of PTPN12 enhanced p130cas phosphorylation and the migratory behavior of a noninvasive parental bladder tumor control (253J). These data show that the metastatic phenotype is accompanied by increases in steady-state H2O2 production that drive promigratory signaling and suggest that antioxidant-based therapeutics may prove useful in limiting bladder tumor invasiveness.

Original languageEnglish (US)
Pages (from-to)303-312
Number of pages10
JournalMolecular Cancer Research
Volume11
Issue number3
DOIs
StatePublished - Mar 1 2013

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Urinary Bladder Neoplasms
Phenotype
Phosphoric Monoester Hydrolases
Crk-Associated Substrate Protein
Phosphorylation
Tumor Cell Line
Sulfhydryl Compounds
Hydrogen Peroxide
Oxidation-Reduction
Cysteine
Actins
Reactive Oxygen Species
Catalytic Domain
Proteins
Antioxidants
Membranes
Therapeutics

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Oncology
  • Cancer Research

Cite this

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title = "Acquisition of the metastatic phenotype is accompanied by H 2O2-dependent activation of the p130cas signaling complex",
abstract = "Reactive oxygen species (ROS) have emerged as cellular signaling molecules and are implicated in metastatic disease by their ability to drive invasion and migration. Here, we define the signaling adaptor protein p130Cas (Crkassociated substrate) as a key redox-responsive molecular trigger that is engaged in highly invasive metastatic bladder tumor cell lines. Endogenous shifts in steady-state hydrogen peroxide (H2O2) that accompany the metastatic phenotype increase p130Cas phosphorylation, membrane recruitment and association with the scaffolding protein Crk, and subsequent Rac1 activation and actin reorganization. Both enzymatic and nonenzymatic scavenging of H 2O2 abrogates p130Cas-dependent signaling and the migratory and invasive activity of the metastatic bladder tumor cells. Disruption of p130Cas attenuates both invasion and migration of the metastatic variant (253J-BV). 253J-BV cells displayed an increase in global thiol oxidation and a concomitant decrease in total phosphatase activity, common target proteins of active-site cysteine oxidation. The dependence of phosphatases on regulation of p130Cas was highlighted when depletion of PTPN12 enhanced p130cas phosphorylation and the migratory behavior of a noninvasive parental bladder tumor control (253J). These data show that the metastatic phenotype is accompanied by increases in steady-state H2O2 production that drive promigratory signaling and suggest that antioxidant-based therapeutics may prove useful in limiting bladder tumor invasiveness.",
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Acquisition of the metastatic phenotype is accompanied by H 2O2-dependent activation of the p130cas signaling complex. / Hempel, Nadine; Bartling, Toni R.; Mian, Badar; Melendez, J. Andres.

In: Molecular Cancer Research, Vol. 11, No. 3, 01.03.2013, p. 303-312.

Research output: Contribution to journalArticle

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