AcsA-AcsB: The core of the cellulose synthase complex from Gluconacetobacter hansenii ATCC23769

John B. McManus, Ying Deng, Nivedita Nagachar, Teh-hui Kao, Ming Tien

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The gram-negative bacterium, Gluconacetobacter hansenii, produces cellulose of exceptionally high crystallinity in comparison to the cellulose of higher plants. This bacterial cellulose is synthesized and extruded into the extracellular medium by the cellulose synthase complex (CSC). The catalytic component of this complex is encoded by the gene AcsAB. However, several other genes are known to encode proteins critical to cellulose synthesis and are likely components of the bacterial CSC. We have purified an active heterodimer AcsA-AcsB from G. hansenii ATCC23769 to homogeneity by two different methods. With the purified protein, we have determined how it is post-translationally processed, forming the active heterodimer AcsA-AcsB. Additionally, we have performed steady-state kinetic studies on the AcsA-AcsB complex. Finally through mutagenesis studies, we have explored the roles of the postulated CSC proteins AcsC, AcsD, and CcpAx.

Original languageEnglish (US)
Pages (from-to)58-65
Number of pages8
JournalEnzyme and Microbial Technology
Volume82
DOIs
StatePublished - Jan 1 2016

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Gluconacetobacter
Cellulose
Genes
Mutagenesis
Proteins
Gram-Negative Bacteria
Bacteria
Kinetics
cellulose synthase

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

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title = "AcsA-AcsB: The core of the cellulose synthase complex from Gluconacetobacter hansenii ATCC23769",
abstract = "The gram-negative bacterium, Gluconacetobacter hansenii, produces cellulose of exceptionally high crystallinity in comparison to the cellulose of higher plants. This bacterial cellulose is synthesized and extruded into the extracellular medium by the cellulose synthase complex (CSC). The catalytic component of this complex is encoded by the gene AcsAB. However, several other genes are known to encode proteins critical to cellulose synthesis and are likely components of the bacterial CSC. We have purified an active heterodimer AcsA-AcsB from G. hansenii ATCC23769 to homogeneity by two different methods. With the purified protein, we have determined how it is post-translationally processed, forming the active heterodimer AcsA-AcsB. Additionally, we have performed steady-state kinetic studies on the AcsA-AcsB complex. Finally through mutagenesis studies, we have explored the roles of the postulated CSC proteins AcsC, AcsD, and CcpAx.",
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AcsA-AcsB : The core of the cellulose synthase complex from Gluconacetobacter hansenii ATCC23769. / McManus, John B.; Deng, Ying; Nagachar, Nivedita; Kao, Teh-hui; Tien, Ming.

In: Enzyme and Microbial Technology, Vol. 82, 01.01.2016, p. 58-65.

Research output: Contribution to journalArticle

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