TY - JOUR
T1 - Action of salicylaldoxime on electron transport reactions, fluorescence yield, and light-induced field changes in spinach chloroplasts
T2 - A new mode of inhibition in photosystem II
AU - Golbeck, John H.
N1 - Funding Information:
The author thanks Dr. Bruno Velthuys for performing the 515-nm absorbance measurement and Dr. Richard Radmer for critically reading the manuscript. This research was supported by grants from the National Science Foundation (PCM 77 20526) and from the U. S. Department of Energy (Contract No. EY-76-C-02-3326-AOOOl).
PY - 1980
Y1 - 1980
N2 - Salicylaldoxime (1–10 mm) inhibits chloroplast electron transport reactions by a reversible and an irreversible modification of photosystem II. The irreversible inhibition correlates with removal of the loosely bound pool of manganese associated with the water-splitting mechanism. The reversible inhibition is characterized by (i) a suppression of artificial donor reactions, (ii) a high initial fluorescence yield, and (iii) a decline in the amplitude of the flash-induced electric field across the membrane. After removal of the inhibitor, the initial fluorescence yield declines to near-control levels, but the variable portion of the fluorescence rise remains missing. Addition of an artificial donor restores the variable fluorescence yield and normal electron transport rates to 2,6-dichlorophenolindophenol. Characteristics of the reversible inhibition suggest that salicylaldoxime causes suppression of photochemical charge separation in photosystem II.
AB - Salicylaldoxime (1–10 mm) inhibits chloroplast electron transport reactions by a reversible and an irreversible modification of photosystem II. The irreversible inhibition correlates with removal of the loosely bound pool of manganese associated with the water-splitting mechanism. The reversible inhibition is characterized by (i) a suppression of artificial donor reactions, (ii) a high initial fluorescence yield, and (iii) a decline in the amplitude of the flash-induced electric field across the membrane. After removal of the inhibitor, the initial fluorescence yield declines to near-control levels, but the variable portion of the fluorescence rise remains missing. Addition of an artificial donor restores the variable fluorescence yield and normal electron transport rates to 2,6-dichlorophenolindophenol. Characteristics of the reversible inhibition suggest that salicylaldoxime causes suppression of photochemical charge separation in photosystem II.
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U2 - 10.1016/0003-9861(80)90450-6
DO - 10.1016/0003-9861(80)90450-6
M3 - Article
C2 - 7458330
AN - SCOPUS:0019278742
SN - 0003-9861
VL - 202
SP - 458
EP - 466
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 2
ER -
