Activatable oligomerizable imaging agents for photoacoustic imaging of furin-like activity in living subjects

Anca Dragulescu-Andrasi, Sri Rajasekhar Kothapalli, Grigory A. Tikhomirov, Jianghong Rao, Sanjiv S. Gambhir

Research output: Contribution to journalArticle

118 Scopus citations

Abstract

Photoacoustic (PA) imaging is continuing to be applied for physiological imaging and more recently for molecular imaging of living subjects. Owing to its high spatial resolution in deep tissues, PA imaging holds great potential for biomedical applications and molecular diagnostics. There is however a lack of probes for targeted PA imaging, especially in the area of enzyme-activatable probes. Here we introduce a molecular probe, which upon proteolytic processing is retained at the site of enzyme activity and provides PA contrast. The probe oligomerizes via a condensation reaction and accumulates in cells and tumors that express the protease. We demonstrate that this probe reports furin and furin-like activity in cells and tumor models by generating a significantly higher PA signal relative to furin-deficient and nontarget controls. This probe could report enzyme activity in living subjects at depths significantly greater than fluorescence imaging probes and has potential for molecular imaging in deep tumors.

Original languageEnglish (US)
Pages (from-to)11015-11022
Number of pages8
JournalJournal of the American Chemical Society
Volume135
Issue number30
DOIs
StatePublished - Jul 31 2013

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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