Activation of neurons in the rostral ventrolateral medulla increases bronchomotor tone in dogs

J. R. Haselton, P. A. Padrid, Marc Kaufman

Research output: Contribution to journalArticle

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Abstract

Previous work from this laboratory has demonstrated that the chemical activation of cell bodies in the caudal ventrolateral medulla of chloralose-anesthetized dogs decreased bronchomotor tone by withdrawing cholinergic input to airway smooth muscle. In the present study we determined the bronchomotor responses to microinjection of DL-homocysteic acid (100 mM; 25-50 nl) into the rostral ventrolateral (RVL) medulla of chloralose-anesthetized dogs. Total lung resistance was used as a functional index of bronchomotor tone. Microinjection of DL-homocysteic acid into the 20 sites located in the lateral aspect of the RVL medulla increased both total lung resistance [from 6.5 ± 0.4 to 9.1 ± 0.8 (SE) cmH2O · 1-1 · s; P < 0.05] and mean arterial pressure (from 125 ± 5 to 148 ± 8 mmHg; P < 0.05). Microinjection of this amino acid into nine sites located in the medial aspect of the RVL medulla increased mean arterial pressure (from 130 ± 6 to 153 ± 6 mmHg; P < 0.05) but had no effect on total lung resistance. We confirmed in three sites that the increase in total lung resistance evoked by microinjection of DL-homocysteic acid was accompanied by an increase in tracheal smooth muscle tension. The increase in total lung resistance evoked by DL-homocysteic acid was not affected by β-adrenergic blockade but was abolished by muscarinic blockade.

Original languageEnglish (US)
Pages (from-to)210-216
Number of pages7
JournalJournal of applied physiology
Volume71
Issue number1
StatePublished - Jan 1 1991

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Microinjections
Dogs
Neurons
Lung
Chloralose
Cholinergic Agents
Smooth Muscle
Arterial Pressure
Muscle Tonus
Adrenergic Agents
Amino Acids
homocysteic acid

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

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title = "Activation of neurons in the rostral ventrolateral medulla increases bronchomotor tone in dogs",
abstract = "Previous work from this laboratory has demonstrated that the chemical activation of cell bodies in the caudal ventrolateral medulla of chloralose-anesthetized dogs decreased bronchomotor tone by withdrawing cholinergic input to airway smooth muscle. In the present study we determined the bronchomotor responses to microinjection of DL-homocysteic acid (100 mM; 25-50 nl) into the rostral ventrolateral (RVL) medulla of chloralose-anesthetized dogs. Total lung resistance was used as a functional index of bronchomotor tone. Microinjection of DL-homocysteic acid into the 20 sites located in the lateral aspect of the RVL medulla increased both total lung resistance [from 6.5 ± 0.4 to 9.1 ± 0.8 (SE) cmH2O · 1-1 · s; P < 0.05] and mean arterial pressure (from 125 ± 5 to 148 ± 8 mmHg; P < 0.05). Microinjection of this amino acid into nine sites located in the medial aspect of the RVL medulla increased mean arterial pressure (from 130 ± 6 to 153 ± 6 mmHg; P < 0.05) but had no effect on total lung resistance. We confirmed in three sites that the increase in total lung resistance evoked by microinjection of DL-homocysteic acid was accompanied by an increase in tracheal smooth muscle tension. The increase in total lung resistance evoked by DL-homocysteic acid was not affected by β-adrenergic blockade but was abolished by muscarinic blockade.",
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Activation of neurons in the rostral ventrolateral medulla increases bronchomotor tone in dogs. / Haselton, J. R.; Padrid, P. A.; Kaufman, Marc.

In: Journal of applied physiology, Vol. 71, No. 1, 01.01.1991, p. 210-216.

Research output: Contribution to journalArticle

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