Active dilation of penetrating arterioles restores red blood cell flux to penumbral neocortex after focal stroke

Andy Y. Shih, Beth Friedman, Patrick J. Drew, Philbert S. Tsai, Patrick D. Lyden, David Kleinfeld

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Pial arterioles actively change diameter to regulate blood flow to the cortex. However, it is unclear whether arteriole reactivity and its homeostatic role of conserving red blood cell (RBC) flux remains intact after a transient period of ischemia. To examine this issue, we measured vasodynamics in pial arteriole networks that overlie the stroke penumbra during transient middle cerebral artery occlusion in rat. In vivo two-photon laser-scanning microscopy was used to obtain direct and repeated measurements of RBC velocity and lumen diameter of individual arterioles, from which the flux of RBCs was calculated. We observed that occlusion altered surface arteriole flow patterns in a manner that ensured undisrupted flow to penetrating arterioles throughout the imaging field. Small-diameter arterioles (23 m), which included 88% of all penetrating arterioles, exhibited robust vasodilation over a 90-min occlusion period. Critically, persistent vasodilation compensated for an incomplete recovery of RBC velocity during reperfusion to enable a complete restoration of postischemic RBC flux. Further, histologic examination of tissue hypoxia suggested re-oxygenation through all cortical layers of the penumbra. These findings indicate that selective reactivity of small pial arterioles is preserved in the stroke penumbra and acts to conserve RBC flux during reperfusion.

Original languageEnglish (US)
Pages (from-to)738-751
Number of pages14
JournalJournal of Cerebral Blood Flow and Metabolism
Volume29
Issue number4
DOIs
StatePublished - Apr 1 2009

Fingerprint

Neocortex
Arterioles
Dilatation
Erythrocytes
Stroke
Vasodilation
Reperfusion
Middle Cerebral Artery Infarction
Photons
Confocal Microscopy
Ischemia

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Shih, Andy Y. ; Friedman, Beth ; Drew, Patrick J. ; Tsai, Philbert S. ; Lyden, Patrick D. ; Kleinfeld, David. / Active dilation of penetrating arterioles restores red blood cell flux to penumbral neocortex after focal stroke. In: Journal of Cerebral Blood Flow and Metabolism. 2009 ; Vol. 29, No. 4. pp. 738-751.
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abstract = "Pial arterioles actively change diameter to regulate blood flow to the cortex. However, it is unclear whether arteriole reactivity and its homeostatic role of conserving red blood cell (RBC) flux remains intact after a transient period of ischemia. To examine this issue, we measured vasodynamics in pial arteriole networks that overlie the stroke penumbra during transient middle cerebral artery occlusion in rat. In vivo two-photon laser-scanning microscopy was used to obtain direct and repeated measurements of RBC velocity and lumen diameter of individual arterioles, from which the flux of RBCs was calculated. We observed that occlusion altered surface arteriole flow patterns in a manner that ensured undisrupted flow to penetrating arterioles throughout the imaging field. Small-diameter arterioles (23 m), which included 88{\%} of all penetrating arterioles, exhibited robust vasodilation over a 90-min occlusion period. Critically, persistent vasodilation compensated for an incomplete recovery of RBC velocity during reperfusion to enable a complete restoration of postischemic RBC flux. Further, histologic examination of tissue hypoxia suggested re-oxygenation through all cortical layers of the penumbra. These findings indicate that selective reactivity of small pial arterioles is preserved in the stroke penumbra and acts to conserve RBC flux during reperfusion.",
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Active dilation of penetrating arterioles restores red blood cell flux to penumbral neocortex after focal stroke. / Shih, Andy Y.; Friedman, Beth; Drew, Patrick J.; Tsai, Philbert S.; Lyden, Patrick D.; Kleinfeld, David.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 29, No. 4, 01.04.2009, p. 738-751.

Research output: Contribution to journalArticle

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