Active role of fatty acid amino acid conjugates in nitrogen metabolism in Spodoptera litura larvae

Naoko Yoshinaga, Takako Aboshi, Hiroaki Abe, Ritsuo Nishida, Hans T. Alborn, James H. Tumlinson, Naoki Mori

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Since the first fatty acid amino acid conjugate (FAC) was isolated from regurgitant of Spodoptera exigua larvae in 1997 [volicitin: N-(17- hydroxylinolenoyl)-L-glutamine], their role as elicitors of induced responses in plants has been well documented. However, studies of the biosyntheses and the physiological role of FACs in the insect have been minimal. By using 14C-labeled glutamine, glutamic acid, and linolenic acid in feeding studies of Spodoptera litura larvae, combined with tissue analyses, we found glutamine in the midgut cells to be a major source for biosynthesis of FACs. Furthermore, 20% of the glutamine moiety of FACs was derived from glutamic acid and ammonia through enzymatic reaction of glutamine synthetase (GS). To determine whether FACs improve GS productivity, we studied nitrogen assimilation efficiency of S. litura larvae fed on artificial diets containing 15NH4Cl and glutamic acid. When the diet was enriched with linolenic acid, the nitrogen assimilation efficiency improved from 40% to >60%. In the lumen, the biosynthesized FACs are hydrolyzed to fatty acids and glutamine, which are reabsorbed into tissues and hemolymph. These results strongly suggested that FACs play an active role in nitrogen assimilation in Lepidoptera larva and that glutamine containing FACs in the gut lumen may function as a form of storage of glutamine, a key compound of nitrogen metabolism.

Original languageEnglish (US)
Pages (from-to)18058-18063
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number46
DOIs
StatePublished - Nov 18 2008

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Spodoptera
Glutamine
Larva
Nitrogen
Fatty Acids
Amino Acids
Glutamic Acid
Glutamate-Ammonia Ligase
alpha-Linolenic Acid
Diet
Nitrogen Compounds
Lepidoptera
Hemolymph
Ammonia
Insects

All Science Journal Classification (ASJC) codes

  • General

Cite this

Yoshinaga, Naoko ; Aboshi, Takako ; Abe, Hiroaki ; Nishida, Ritsuo ; Alborn, Hans T. ; Tumlinson, James H. ; Mori, Naoki. / Active role of fatty acid amino acid conjugates in nitrogen metabolism in Spodoptera litura larvae. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 46. pp. 18058-18063.
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Active role of fatty acid amino acid conjugates in nitrogen metabolism in Spodoptera litura larvae. / Yoshinaga, Naoko; Aboshi, Takako; Abe, Hiroaki; Nishida, Ritsuo; Alborn, Hans T.; Tumlinson, James H.; Mori, Naoki.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 46, 18.11.2008, p. 18058-18063.

Research output: Contribution to journalArticle

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AU - Yoshinaga, Naoko

AU - Aboshi, Takako

AU - Abe, Hiroaki

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