Activity dependent protein degradation is critical for the formation and stability of fear memory in the amygdala

Timothy J. Jarome, Craig T. Werner, Janine Kwapis, Fred J. Helmstetter

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Protein degradation through the ubiquitin-proteasome system [UPS] plays a critical role in some forms of synaptic plasticity. However, its role in memory formation in the amygdala, a site critical for the formation of fear memories, currently remains unknown. Here we provide the first evidence that protein degradation through the UPS is critically engaged at amygdala synapses during memory formation and retrieval. Fear conditioning results in NMDA-dependent increases in degradation-specific polyubiquitination in the amygdala, targeting proteins involved in translational control and synaptic structure and blocking the degradation of these proteins significantly impairs long-term memory. Furthermore, retrieval of fear memory results in a second wave of NMDA-dependent polyubiquitination that targets proteins involved in translational silencing and synaptic structure and is critical for memory updating following recall. These results indicate that UPS-mediated protein degradation is a major regulator of synaptic plasticity necessary for the formation and stability of long-term memories at amygdala synapses.

Original languageEnglish (US)
Article numbere24349
JournalPloS one
Volume6
Issue number9
DOIs
StatePublished - Sep 22 2011

Fingerprint

amygdala
protein degradation
Amygdala
fearfulness
Proteolysis
Fear
Data storage equipment
Degradation
Proteasome Endopeptidase Complex
Ubiquitin
Neuronal Plasticity
proteasome endopeptidase complex
Long-Term Memory
ubiquitin
N-Methylaspartate
Proteins
Synapses
synapse
Plasticity
Protein Transport

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Jarome, Timothy J. ; Werner, Craig T. ; Kwapis, Janine ; Helmstetter, Fred J. / Activity dependent protein degradation is critical for the formation and stability of fear memory in the amygdala. In: PloS one. 2011 ; Vol. 6, No. 9.
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Activity dependent protein degradation is critical for the formation and stability of fear memory in the amygdala. / Jarome, Timothy J.; Werner, Craig T.; Kwapis, Janine; Helmstetter, Fred J.

In: PloS one, Vol. 6, No. 9, e24349, 22.09.2011.

Research output: Contribution to journalArticle

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