Activity of external hyphae of vesicular-arbuscular mycorrhizal fungi

David M. Sylvia

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

A method to quantify active external hyphae of VAM fungi on root surfaces and in soil is described. Hyphae attached to roots or extracted from soil are incubated in a solution containing indonitrotetrazolium and NADH and counter-stained with trypan blue. The method allows for the assessment of growth VAM fungi in soil. Pot cultures of Paspalum notatum were inoculated with Clomus mosseae or G. intraradices and were grown in a glasshouse for 13 wk. Greater than 96% of the hyphae attached to roots were active from week 3 to 13. The proportion of active hyphae extracted from soil ranged from 0 to 32% during the 13 wk period, but increased dramatically between 6 and 9 wk. The production of active external hyphae lagged behind root colonization by several weeks. The importance of hyphal activity in relation to nutrient uptake and translocation by VAM fungi is discussed.

Original languageEnglish (US)
Pages (from-to)39-42
Number of pages4
JournalSoil Biology and Biochemistry
Volume20
Issue number1
DOIs
StatePublished - Jan 1 1988

Fingerprint

Hyphae
hyphae
mycorrhizal fungi
Fungi
fungus
Soil
soil
Paspalum
root colonization
nutrient uptake
Paspalum notatum
pot culture
fungi
Trypan Blue
translocation
soil fungi
NAD
greenhouses
Growth
methodology

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Soil Science

Cite this

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abstract = "A method to quantify active external hyphae of VAM fungi on root surfaces and in soil is described. Hyphae attached to roots or extracted from soil are incubated in a solution containing indonitrotetrazolium and NADH and counter-stained with trypan blue. The method allows for the assessment of growth VAM fungi in soil. Pot cultures of Paspalum notatum were inoculated with Clomus mosseae or G. intraradices and were grown in a glasshouse for 13 wk. Greater than 96{\%} of the hyphae attached to roots were active from week 3 to 13. The proportion of active hyphae extracted from soil ranged from 0 to 32{\%} during the 13 wk period, but increased dramatically between 6 and 9 wk. The production of active external hyphae lagged behind root colonization by several weeks. The importance of hyphal activity in relation to nutrient uptake and translocation by VAM fungi is discussed.",
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Activity of external hyphae of vesicular-arbuscular mycorrhizal fungi. / Sylvia, David M.

In: Soil Biology and Biochemistry, Vol. 20, No. 1, 01.01.1988, p. 39-42.

Research output: Contribution to journalArticle

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