Activity, structure, and stratification of membrane-attached methanotrophic biofilms cometabolically degrading trichloroethylene

Lee W. Clapp, John M. Regan, Firdaus Ali, Jack D. Newman, Jae K. Park, Daniel R. Noguera

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A membrane-attached methanotrophic biofilm reactor was developed for the cometabolic degradation of trichloroethylene (TCE). In this reactor, CH4 and O2 are supplied to the interior of the biofilm through the membrane, while TCE-contaminated water is supplied to the exterior, creating a 'counter-diffusional' effect that minimizes competitive inhibition between TCE and CH4. In addition, this novel design provides 100% CH4 and O2 transfer efficiencies, promotes the development of a thick biofilm, and minimizes the negative effects of TCE byproduct toxicity. The reactor sustained 80-90% TCE removals at TCE loading rates ranging from 100-320 μmol/m2/d. Chloride mass balances demonstrated that 60-80% of the TCE removed was mineralized. The maximum TCE transformation yield was 1.8 mmol of TCE removed per mole of CH4 utilized, although higher transformation yields are expected at higher TCE loading rates. The CH4 utilization rate was 0.20 mol/m2/d. Scanning electron microscopy (SEM) revealed a dense biofilm with a thickness of at least 400 μm. SEM and transmission electron microscopy (TEM) analyses indicated that the 'holdfast' material associated with rosette formation in planktonic Methylosinus trichosporium OB3b (M.t. OB3b) cells might also contribute to pure-culture biofilm development. In addition, fimbriae-like structures not commonly associated with methanotrophic bacteria were observed in pure-culture M.t. OB3b biofilms. Finally, fluorescent in situ hybridization (FISH) analyses showed the presence of discrete microcolonies of serine-pathway methanotrophs within mixed-culture biofilms.

Original languageEnglish (US)
Pages (from-to)153-161
Number of pages9
JournalWater Science and Technology
Volume39
Issue number7
DOIs
StatePublished - Jun 5 1999

Fingerprint

Trichloroethylene
Biofilms
trichloroethylene
biofilm
stratification
membrane
Membranes
scanning electron microscopy
Scanning electron microscopy
Byproducts
Toxicity
transmission electron microscopy
mass balance
Bacteria
chloride
toxicity
Transmission electron microscopy
Degradation
degradation

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Water Science and Technology

Cite this

Clapp, Lee W. ; Regan, John M. ; Ali, Firdaus ; Newman, Jack D. ; Park, Jae K. ; Noguera, Daniel R. / Activity, structure, and stratification of membrane-attached methanotrophic biofilms cometabolically degrading trichloroethylene. In: Water Science and Technology. 1999 ; Vol. 39, No. 7. pp. 153-161.
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abstract = "A membrane-attached methanotrophic biofilm reactor was developed for the cometabolic degradation of trichloroethylene (TCE). In this reactor, CH4 and O2 are supplied to the interior of the biofilm through the membrane, while TCE-contaminated water is supplied to the exterior, creating a 'counter-diffusional' effect that minimizes competitive inhibition between TCE and CH4. In addition, this novel design provides 100{\%} CH4 and O2 transfer efficiencies, promotes the development of a thick biofilm, and minimizes the negative effects of TCE byproduct toxicity. The reactor sustained 80-90{\%} TCE removals at TCE loading rates ranging from 100-320 μmol/m2/d. Chloride mass balances demonstrated that 60-80{\%} of the TCE removed was mineralized. The maximum TCE transformation yield was 1.8 mmol of TCE removed per mole of CH4 utilized, although higher transformation yields are expected at higher TCE loading rates. The CH4 utilization rate was 0.20 mol/m2/d. Scanning electron microscopy (SEM) revealed a dense biofilm with a thickness of at least 400 μm. SEM and transmission electron microscopy (TEM) analyses indicated that the 'holdfast' material associated with rosette formation in planktonic Methylosinus trichosporium OB3b (M.t. OB3b) cells might also contribute to pure-culture biofilm development. In addition, fimbriae-like structures not commonly associated with methanotrophic bacteria were observed in pure-culture M.t. OB3b biofilms. Finally, fluorescent in situ hybridization (FISH) analyses showed the presence of discrete microcolonies of serine-pathway methanotrophs within mixed-culture biofilms.",
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Activity, structure, and stratification of membrane-attached methanotrophic biofilms cometabolically degrading trichloroethylene. / Clapp, Lee W.; Regan, John M.; Ali, Firdaus; Newman, Jack D.; Park, Jae K.; Noguera, Daniel R.

In: Water Science and Technology, Vol. 39, No. 7, 05.06.1999, p. 153-161.

Research output: Contribution to journalArticle

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T1 - Activity, structure, and stratification of membrane-attached methanotrophic biofilms cometabolically degrading trichloroethylene

AU - Clapp, Lee W.

AU - Regan, John M.

AU - Ali, Firdaus

AU - Newman, Jack D.

AU - Park, Jae K.

AU - Noguera, Daniel R.

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N2 - A membrane-attached methanotrophic biofilm reactor was developed for the cometabolic degradation of trichloroethylene (TCE). In this reactor, CH4 and O2 are supplied to the interior of the biofilm through the membrane, while TCE-contaminated water is supplied to the exterior, creating a 'counter-diffusional' effect that minimizes competitive inhibition between TCE and CH4. In addition, this novel design provides 100% CH4 and O2 transfer efficiencies, promotes the development of a thick biofilm, and minimizes the negative effects of TCE byproduct toxicity. The reactor sustained 80-90% TCE removals at TCE loading rates ranging from 100-320 μmol/m2/d. Chloride mass balances demonstrated that 60-80% of the TCE removed was mineralized. The maximum TCE transformation yield was 1.8 mmol of TCE removed per mole of CH4 utilized, although higher transformation yields are expected at higher TCE loading rates. The CH4 utilization rate was 0.20 mol/m2/d. Scanning electron microscopy (SEM) revealed a dense biofilm with a thickness of at least 400 μm. SEM and transmission electron microscopy (TEM) analyses indicated that the 'holdfast' material associated with rosette formation in planktonic Methylosinus trichosporium OB3b (M.t. OB3b) cells might also contribute to pure-culture biofilm development. In addition, fimbriae-like structures not commonly associated with methanotrophic bacteria were observed in pure-culture M.t. OB3b biofilms. Finally, fluorescent in situ hybridization (FISH) analyses showed the presence of discrete microcolonies of serine-pathway methanotrophs within mixed-culture biofilms.

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