Acute removal of common sepsis mediators does not explain the effects of extracorporeal blood purification in experimental sepsis

Zhi Yong Peng, Hong Zhi Wang, Melinda J. Carter, Morgan V. Dileo, Jeffery V. Bishop, Fei Hu Zhou, Xiao Yan Wen, Thomas Rimmelé, Kai Singbartl, William J. Federspiel, Gilles Clermont, John A. Kellum

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Abstract

The effect of extracorporeal blood purification on clinical outcomes in sepsis is assumed to be related to modulation of plasma cytokine concentrations. To test this hypothesis directly, we treated rats that had a cecal ligation followed by puncture (a standard model of sepsis) with a modest dose of extracorporeal blood purification that did not result in acute changes in a panel of common cytokines associated with inflammation (TNF-α, IL-1Β, IL-6, and IL-10). Pre- and immediate post-treatment levels of these cytokines were unchanged compared to the sham therapy of extracorporeal circulation without blood purifying sorbent. The overall survival to 7 days, however, was significantly better in animals that received extracorporeal blood purification compared to those with a sham procedure. This panel of common plasma cytokines along with alanine aminotransferase and creatinine was significantly lower 72 h following extracorporeal blood purification compared to sham-treated rats. Thus, the effects of this procedure on organ function and survival do not appear to be due solely to immediate changes in the usual measured circulating cytokines. These results may have important implications for the design and conduct of future trials in sepsis including defining alternative targets for extracorporeal blood purification and other therapies.

Original languageEnglish (US)
Pages (from-to)363-369
Number of pages7
JournalKidney International
Volume81
Issue number4
DOIs
StatePublished - Feb 2 2012

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Sepsis
Cytokines
Tissue Survival
Extracorporeal Circulation
Alanine Transaminase
Interleukin-1
Punctures
Interleukin-10
Ligation
Interleukin-6
Creatinine
Therapeutics
Inflammation

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

Peng, Z. Y., Wang, H. Z., Carter, M. J., Dileo, M. V., Bishop, J. V., Zhou, F. H., ... Kellum, J. A. (2012). Acute removal of common sepsis mediators does not explain the effects of extracorporeal blood purification in experimental sepsis. Kidney International, 81(4), 363-369. https://doi.org/10.1038/ki.2011.320
Peng, Zhi Yong ; Wang, Hong Zhi ; Carter, Melinda J. ; Dileo, Morgan V. ; Bishop, Jeffery V. ; Zhou, Fei Hu ; Wen, Xiao Yan ; Rimmelé, Thomas ; Singbartl, Kai ; Federspiel, William J. ; Clermont, Gilles ; Kellum, John A. / Acute removal of common sepsis mediators does not explain the effects of extracorporeal blood purification in experimental sepsis. In: Kidney International. 2012 ; Vol. 81, No. 4. pp. 363-369.
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Peng, ZY, Wang, HZ, Carter, MJ, Dileo, MV, Bishop, JV, Zhou, FH, Wen, XY, Rimmelé, T, Singbartl, K, Federspiel, WJ, Clermont, G & Kellum, JA 2012, 'Acute removal of common sepsis mediators does not explain the effects of extracorporeal blood purification in experimental sepsis', Kidney International, vol. 81, no. 4, pp. 363-369. https://doi.org/10.1038/ki.2011.320

Acute removal of common sepsis mediators does not explain the effects of extracorporeal blood purification in experimental sepsis. / Peng, Zhi Yong; Wang, Hong Zhi; Carter, Melinda J.; Dileo, Morgan V.; Bishop, Jeffery V.; Zhou, Fei Hu; Wen, Xiao Yan; Rimmelé, Thomas; Singbartl, Kai; Federspiel, William J.; Clermont, Gilles; Kellum, John A.

In: Kidney International, Vol. 81, No. 4, 02.02.2012, p. 363-369.

Research output: Contribution to journalArticle

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AU - Zhou, Fei Hu

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AU - Singbartl, Kai

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AU - Clermont, Gilles

AU - Kellum, John A.

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