Using a nonhuman primate model, we examined the mechanisms by which acute social stress inhibits the ability of NK cells to form conjugates with, and lyse target cells. We examined the expression and role of the primary NK cell adhesion molecules, CD2 and LFA-1, in mediating conjugation to target cells. Acute stress induced a decrease in NK cell expression of CD2 (17 ± 3%); and to a lesser degree induced a decrease in expression of LFA-1 (CD11a: 8 ± 3%; CD18: 7 ± 3%). Antibody blocking studies indicated that anti-LFA-1 significantly inhibited NK cell conjugate formation and cytotoxicity in both control (~40% and ~50%, respectively) and stressed (~20% and ~45%, respectively) conditions. However, anti-CD2 blocked conjugation and cytotoxicity in the control condition by ~50%, but had no capacity to further affect the inhibition of conjugation or cytotoxicity of NK cells induced by acute stress. These data indicate that there are differential effects of acute stress on the expression and function of LFA-1 and CD2, and that the stress-induced inhibition of NK cell adhesion and cytotoxicity is dependent upon modulation of adhesion and/or signalling through CD2.
All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Clinical Neurology