Acute stress impairs NK cell adhesion and cytotoxicity through CD2, but not LFA-1

Connie Jo Rogers, Cynthia S. Brissette-Storkus, William H. Chambers, Judy L. Cameron

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Using a nonhuman primate model, we examined the mechanisms by which acute social stress inhibits the ability of NK cells to form conjugates with, and lyse target cells. We examined the expression and role of the primary NK cell adhesion molecules, CD2 and LFA-1, in mediating conjugation to target cells. Acute stress induced a decrease in NK cell expression of CD2 (17 ± 3%); and to a lesser degree induced a decrease in expression of LFA-1 (CD11a: 8 ± 3%; CD18: 7 ± 3%). Antibody blocking studies indicated that anti-LFA-1 significantly inhibited NK cell conjugate formation and cytotoxicity in both control (~40% and ~50%, respectively) and stressed (~20% and ~45%, respectively) conditions. However, anti-CD2 blocked conjugation and cytotoxicity in the control condition by ~50%, but had no capacity to further affect the inhibition of conjugation or cytotoxicity of NK cells induced by acute stress. These data indicate that there are differential effects of acute stress on the expression and function of LFA-1 and CD2, and that the stress-induced inhibition of NK cell adhesion and cytotoxicity is dependent upon modulation of adhesion and/or signalling through CD2.

Original languageEnglish (US)
Pages (from-to)230-241
Number of pages12
JournalJournal of Neuroimmunology
Volume99
Issue number2
DOIs
StatePublished - Oct 29 1999

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Lymphocyte Function-Associated Antigen-1
Cell Adhesion
Natural Killer Cells
Blocking Antibodies
Cell Adhesion Molecules
Primates

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Cite this

Rogers, Connie Jo ; Brissette-Storkus, Cynthia S. ; Chambers, William H. ; Cameron, Judy L. / Acute stress impairs NK cell adhesion and cytotoxicity through CD2, but not LFA-1. In: Journal of Neuroimmunology. 1999 ; Vol. 99, No. 2. pp. 230-241.
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abstract = "Using a nonhuman primate model, we examined the mechanisms by which acute social stress inhibits the ability of NK cells to form conjugates with, and lyse target cells. We examined the expression and role of the primary NK cell adhesion molecules, CD2 and LFA-1, in mediating conjugation to target cells. Acute stress induced a decrease in NK cell expression of CD2 (17 ± 3{\%}); and to a lesser degree induced a decrease in expression of LFA-1 (CD11a: 8 ± 3{\%}; CD18: 7 ± 3{\%}). Antibody blocking studies indicated that anti-LFA-1 significantly inhibited NK cell conjugate formation and cytotoxicity in both control (~40{\%} and ~50{\%}, respectively) and stressed (~20{\%} and ~45{\%}, respectively) conditions. However, anti-CD2 blocked conjugation and cytotoxicity in the control condition by ~50{\%}, but had no capacity to further affect the inhibition of conjugation or cytotoxicity of NK cells induced by acute stress. These data indicate that there are differential effects of acute stress on the expression and function of LFA-1 and CD2, and that the stress-induced inhibition of NK cell adhesion and cytotoxicity is dependent upon modulation of adhesion and/or signalling through CD2.",
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Acute stress impairs NK cell adhesion and cytotoxicity through CD2, but not LFA-1. / Rogers, Connie Jo; Brissette-Storkus, Cynthia S.; Chambers, William H.; Cameron, Judy L.

In: Journal of Neuroimmunology, Vol. 99, No. 2, 29.10.1999, p. 230-241.

Research output: Contribution to journalArticle

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