Adenosine receptor expression in the adult zebrafish retina

Stephanie Grillo, Dillon S. McDevitt, Matthew G. Voas, Amanda S. Khan, Michael A. Grillo, Salvatore Stella

Research output: Contribution to journalArticle

Abstract

Adenosine is an endogenous nucleoside in the central nervous system that acts on adenosine receptors. These are G protein-coupled receptors that have four known subtypes: A1, A2A, A2B, and A3 receptors. In the present study, we aimed to map the location of the adenosine receptor subtypes in adult wild-type zebrafish retina using in situ hybridization and immunohistochemistry. A1R, A2AR, and A2BR mRNA were detected in the ganglion cell layer (GCL), the inner nuclear layer (INL), the outer nuclear layer (ONL), and the outer segment (OS). A3R mRNA was detected in the GCL, ONL, and OS. A1R-immunoreactivity was expressed as puncta in the INL and in the outer plexiform layer (OPL). A1Rs were located within the cone pedicle and contiguous to horizontal cell tips in the OPL. A2AR-immunoreactivity was expressed as puncta in the GCL, inner plexiform layer (IPL), INL, and outer retina. A2AR puncta in the outer retina were situated around the ellipsoids and nuclei of cones, and weakly around the rod nuclei. A1Rs and A2ARs were clustered around ON cone bipolar cell terminals and present in the OFF lamina of the INL but were not expressed on mixed rod/cone response bipolar cell terminals. A2BR-immunoreactivity was mainly localized to the Müller cells, while A3Rs were found to be expressed in retinal ganglion cells of the GCL, INL, ONL, and OS. In summary, all four adenosine receptor subtypes were localized in the zebrafish retina and are in agreement with expression patterns shown in retinas from other species.

Original languageEnglish (US)
Pages (from-to)327-342
Number of pages16
JournalPurinergic Signalling
Volume15
Issue number3
DOIs
StatePublished - Sep 1 2019

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Purinergic P1 Receptors
Zebrafish
Retina
Ganglia
Nuclear Lamina
Messenger RNA
Vertebrate Photoreceptor Cells
G-Protein-Coupled Receptors
Nucleosides
Adenosine
In Situ Hybridization
Central Nervous System
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Grillo, Stephanie ; McDevitt, Dillon S. ; Voas, Matthew G. ; Khan, Amanda S. ; Grillo, Michael A. ; Stella, Salvatore. / Adenosine receptor expression in the adult zebrafish retina. In: Purinergic Signalling. 2019 ; Vol. 15, No. 3. pp. 327-342.
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Adenosine receptor expression in the adult zebrafish retina. / Grillo, Stephanie; McDevitt, Dillon S.; Voas, Matthew G.; Khan, Amanda S.; Grillo, Michael A.; Stella, Salvatore.

In: Purinergic Signalling, Vol. 15, No. 3, 01.09.2019, p. 327-342.

Research output: Contribution to journalArticle

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T1 - Adenosine receptor expression in the adult zebrafish retina

AU - Grillo, Stephanie

AU - McDevitt, Dillon S.

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AU - Khan, Amanda S.

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AU - Stella, Salvatore

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AB - Adenosine is an endogenous nucleoside in the central nervous system that acts on adenosine receptors. These are G protein-coupled receptors that have four known subtypes: A1, A2A, A2B, and A3 receptors. In the present study, we aimed to map the location of the adenosine receptor subtypes in adult wild-type zebrafish retina using in situ hybridization and immunohistochemistry. A1R, A2AR, and A2BR mRNA were detected in the ganglion cell layer (GCL), the inner nuclear layer (INL), the outer nuclear layer (ONL), and the outer segment (OS). A3R mRNA was detected in the GCL, ONL, and OS. A1R-immunoreactivity was expressed as puncta in the INL and in the outer plexiform layer (OPL). A1Rs were located within the cone pedicle and contiguous to horizontal cell tips in the OPL. A2AR-immunoreactivity was expressed as puncta in the GCL, inner plexiform layer (IPL), INL, and outer retina. A2AR puncta in the outer retina were situated around the ellipsoids and nuclei of cones, and weakly around the rod nuclei. A1Rs and A2ARs were clustered around ON cone bipolar cell terminals and present in the OFF lamina of the INL but were not expressed on mixed rod/cone response bipolar cell terminals. A2BR-immunoreactivity was mainly localized to the Müller cells, while A3Rs were found to be expressed in retinal ganglion cells of the GCL, INL, ONL, and OS. In summary, all four adenosine receptor subtypes were localized in the zebrafish retina and are in agreement with expression patterns shown in retinas from other species.

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