Adhesion and cytokine production by monocytes on poly(2‐methacryloyloxyethyl phosphorylcholine‐co‐alkyl methacrylate)‐coated polymers

K. M. Defife, Jong Yun, A. Azeez, S. Stack, K. Ishihara, N. Nakabayashi, E. Colton, J. M. Anderson

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Human monocytes isolated from peripheral venous blood were assayed for their ability to adhere to various polymers. The culture supernatants were also assayed for the cytokines, interleukin‐1β (IL‐1β), interleukin‐6 (IL‐6), and tumor necrosis factor‐α (TNF‐α). The polymers evaluated for adherence and cytokine production included Pellethane®, polyethylene and poly[n‐butyl methacrylate (BMA)] coated with poly[2‐methacryloyloxyethyl phosphorylcholine (MPC)‐co‐alkyl methacrylate] copolymers. In some experiments the test polymers were adsorbed with fibrinogen or IgG prior to the addition of monocytes. MPC copolymer‐coated materials inhibited monocyte and macrophage adhesion after 1 and 8 days of culture relative to corresponding uncoated polymers and tissue culture polystyrene (TCPS). The degree of inhibition by coated Pellethane compared to uncoated Pellethane was the greatest, while inhibition of adhesion by coated poly(BMA) was the least compared to uncoated poly(BMA). However, adhesion was significantly decreased on both coated and uncoated poly(BMA) by day 8. While IL‐1β, IL‐6, and TNF‐α release was variably influenced by polymer coating, release was consitently inhibited relative to TCPS on day 1. However, cytokine production was not inhibited compared to corresponding uncoated polymers on day 1. With or without protein preadsorption, IL‐1β release was not detectable in the supernatants of any polymer on day 8, IL‐6 production was diminished on day 8, and TNF‐α production was sustained on day 8. Overall, MPC copolymercoated and uncoated poly(BMA) were the least stimulating, while TCPS was the most stimulating. These studies suggest that MPC copolymers may improve the cell adhesion‐resistant properties of a biomaterial while variably influencing the activation of cells which may contact the coated surface. © 1995 John Wiley & Sons, Inc.

Original languageEnglish (US)
Pages (from-to)431-439
Number of pages9
JournalJournal of Biomedical Materials Research
Volume29
Issue number4
DOIs
StatePublished - Jan 1 1995

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Methacrylates
Polymers
Adhesion
Cytokines
Tissue culture
Polyurethanes
Polystyrenes
Tumor Necrosis Factor-alpha
Copolymers
Macrophages
Phosphorylcholine
Cell culture
Biomaterials
Biocompatible Materials
Polyethylene
Polyethylenes
Fibrinogen
Blood
Chemical activation
Immunoglobulin G

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

Cite this

Defife, K. M. ; Yun, Jong ; Azeez, A. ; Stack, S. ; Ishihara, K. ; Nakabayashi, N. ; Colton, E. ; Anderson, J. M. / Adhesion and cytokine production by monocytes on poly(2‐methacryloyloxyethyl phosphorylcholine‐co‐alkyl methacrylate)‐coated polymers. In: Journal of Biomedical Materials Research. 1995 ; Vol. 29, No. 4. pp. 431-439.
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abstract = "Human monocytes isolated from peripheral venous blood were assayed for their ability to adhere to various polymers. The culture supernatants were also assayed for the cytokines, interleukin‐1β (IL‐1β), interleukin‐6 (IL‐6), and tumor necrosis factor‐α (TNF‐α). The polymers evaluated for adherence and cytokine production included Pellethane{\circledR}, polyethylene and poly[n‐butyl methacrylate (BMA)] coated with poly[2‐methacryloyloxyethyl phosphorylcholine (MPC)‐co‐alkyl methacrylate] copolymers. In some experiments the test polymers were adsorbed with fibrinogen or IgG prior to the addition of monocytes. MPC copolymer‐coated materials inhibited monocyte and macrophage adhesion after 1 and 8 days of culture relative to corresponding uncoated polymers and tissue culture polystyrene (TCPS). The degree of inhibition by coated Pellethane compared to uncoated Pellethane was the greatest, while inhibition of adhesion by coated poly(BMA) was the least compared to uncoated poly(BMA). However, adhesion was significantly decreased on both coated and uncoated poly(BMA) by day 8. While IL‐1β, IL‐6, and TNF‐α release was variably influenced by polymer coating, release was consitently inhibited relative to TCPS on day 1. However, cytokine production was not inhibited compared to corresponding uncoated polymers on day 1. With or without protein preadsorption, IL‐1β release was not detectable in the supernatants of any polymer on day 8, IL‐6 production was diminished on day 8, and TNF‐α production was sustained on day 8. Overall, MPC copolymercoated and uncoated poly(BMA) were the least stimulating, while TCPS was the most stimulating. These studies suggest that MPC copolymers may improve the cell adhesion‐resistant properties of a biomaterial while variably influencing the activation of cells which may contact the coated surface. {\circledC} 1995 John Wiley & Sons, Inc.",
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Adhesion and cytokine production by monocytes on poly(2‐methacryloyloxyethyl phosphorylcholine‐co‐alkyl methacrylate)‐coated polymers. / Defife, K. M.; Yun, Jong; Azeez, A.; Stack, S.; Ishihara, K.; Nakabayashi, N.; Colton, E.; Anderson, J. M.

In: Journal of Biomedical Materials Research, Vol. 29, No. 4, 01.01.1995, p. 431-439.

Research output: Contribution to journalArticle

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T1 - Adhesion and cytokine production by monocytes on poly(2‐methacryloyloxyethyl phosphorylcholine‐co‐alkyl methacrylate)‐coated polymers

AU - Defife, K. M.

AU - Yun, Jong

AU - Azeez, A.

AU - Stack, S.

AU - Ishihara, K.

AU - Nakabayashi, N.

AU - Colton, E.

AU - Anderson, J. M.

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N2 - Human monocytes isolated from peripheral venous blood were assayed for their ability to adhere to various polymers. The culture supernatants were also assayed for the cytokines, interleukin‐1β (IL‐1β), interleukin‐6 (IL‐6), and tumor necrosis factor‐α (TNF‐α). The polymers evaluated for adherence and cytokine production included Pellethane®, polyethylene and poly[n‐butyl methacrylate (BMA)] coated with poly[2‐methacryloyloxyethyl phosphorylcholine (MPC)‐co‐alkyl methacrylate] copolymers. In some experiments the test polymers were adsorbed with fibrinogen or IgG prior to the addition of monocytes. MPC copolymer‐coated materials inhibited monocyte and macrophage adhesion after 1 and 8 days of culture relative to corresponding uncoated polymers and tissue culture polystyrene (TCPS). The degree of inhibition by coated Pellethane compared to uncoated Pellethane was the greatest, while inhibition of adhesion by coated poly(BMA) was the least compared to uncoated poly(BMA). However, adhesion was significantly decreased on both coated and uncoated poly(BMA) by day 8. While IL‐1β, IL‐6, and TNF‐α release was variably influenced by polymer coating, release was consitently inhibited relative to TCPS on day 1. However, cytokine production was not inhibited compared to corresponding uncoated polymers on day 1. With or without protein preadsorption, IL‐1β release was not detectable in the supernatants of any polymer on day 8, IL‐6 production was diminished on day 8, and TNF‐α production was sustained on day 8. Overall, MPC copolymercoated and uncoated poly(BMA) were the least stimulating, while TCPS was the most stimulating. These studies suggest that MPC copolymers may improve the cell adhesion‐resistant properties of a biomaterial while variably influencing the activation of cells which may contact the coated surface. © 1995 John Wiley & Sons, Inc.

AB - Human monocytes isolated from peripheral venous blood were assayed for their ability to adhere to various polymers. The culture supernatants were also assayed for the cytokines, interleukin‐1β (IL‐1β), interleukin‐6 (IL‐6), and tumor necrosis factor‐α (TNF‐α). The polymers evaluated for adherence and cytokine production included Pellethane®, polyethylene and poly[n‐butyl methacrylate (BMA)] coated with poly[2‐methacryloyloxyethyl phosphorylcholine (MPC)‐co‐alkyl methacrylate] copolymers. In some experiments the test polymers were adsorbed with fibrinogen or IgG prior to the addition of monocytes. MPC copolymer‐coated materials inhibited monocyte and macrophage adhesion after 1 and 8 days of culture relative to corresponding uncoated polymers and tissue culture polystyrene (TCPS). The degree of inhibition by coated Pellethane compared to uncoated Pellethane was the greatest, while inhibition of adhesion by coated poly(BMA) was the least compared to uncoated poly(BMA). However, adhesion was significantly decreased on both coated and uncoated poly(BMA) by day 8. While IL‐1β, IL‐6, and TNF‐α release was variably influenced by polymer coating, release was consitently inhibited relative to TCPS on day 1. However, cytokine production was not inhibited compared to corresponding uncoated polymers on day 1. With or without protein preadsorption, IL‐1β release was not detectable in the supernatants of any polymer on day 8, IL‐6 production was diminished on day 8, and TNF‐α production was sustained on day 8. Overall, MPC copolymercoated and uncoated poly(BMA) were the least stimulating, while TCPS was the most stimulating. These studies suggest that MPC copolymers may improve the cell adhesion‐resistant properties of a biomaterial while variably influencing the activation of cells which may contact the coated surface. © 1995 John Wiley & Sons, Inc.

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