TY - CHAP
T1 - Adhesion and Signaling of Tumor Cells to Leukocytes and Endothelium in Cancer Metastasis
AU - Dong, Cheng
N1 - Funding Information:
The author thanks his former and current graduate students for their outstanding contributions to the work presented in this chapter, especially Dr. Margret Slattery, Dr. Shile Liang, Dr. Hsin H. Peng, Dr. Meghan Hoskins, Ms. Payal Khanna and Ms. Tara Yunkunis. He greatly acknowledges Dr. Gavin Robertson, Dr. Arati Sharma, Dr. Avery August, and Dr. Robert Kunz (all from Penn State University), who have contributed significant collaboration to the work. Gratitude also extends to Dr. Meenhard Herlyn (Wistar Institute, Philadelphia, PA, USA), Dr. Danny Welch (University of Alabama, Birmingham), and Dr. Scott Simon (UC Davis, CA, USA) for kindly providing cell lines and reagents. This work was supported in part by the National Institutes of Health grants CA97306 and CA-125707, the National Science Foundation grant CBET-0729091, the Johnson & Johnson Innovative Technology, and the PA Dept. of Health Research Fund SAP #41000-26343.
Publisher Copyright:
© 2010, Springer-Verlag Berlin Heidelberg.
PY - 2011
Y1 - 2011
N2 - Heterotypic cell–cell adhesion in the near wall region under dynamic shear forces has been studied. In particular, we focus on neutrophil (PMN)–melanoma cell emboli formation in a non-linear shear flow and subsequent tethering to the vascular endothelium (EC) as a result of cell–cell aggregation. The extent of tumor cell adhesion to a vessel wall is governed by the kinetic formation/disruption of receptor–ligand bonds, soluble signaling proteins within the tumor microenvironment, and the hydrodynamic shear within the circulation. Upon tumor cell arrest on the endothelium, retraction of EC during tumor cell extravasation occurs due to the disruption of intercellular channels or disassembly of the vascular endothelial (VE)-cadherin homodimers that allow the passage of soluble proteins and cells. Preliminary studies have found tumor-elicited PMNs increase melanoma cell extravasation, which involves PMNs tethering on the EC and subsequently capturing/maintaining melanoma cells in close proximity to the EC. Results have indicated a novel finding that PMN-facilitated melanoma cell arrest on the EC is mediated by binding between the intercellular adhesion molecule (ICAM)-1 (expressing on both melanoma cells and ECs) and β2 integrins on PMNs, influenced by tumor-induced inflammatory cytokines, e.g., interleukin (IL)-8, and hydrodynamic shear rates. Furthermore, the adherens junctions in terms of VE-cadherin are regulated by endothelial mitogen activated protein kinases (MAPK) in response to tumor cell adhesion to the EC, as well as to IL-8 and several other soluble signaling proteins within the tumor microenvironment. These studies will yield new evidence for the complex role of hemodynamics, protein signaling, and heterotypic cell adhesion in the recruitment of metastatic cancer cells to the EC in the microcirculation during metastasis, which will be significant in fostering new cross-disciplinary approaches to cancer treatment.
AB - Heterotypic cell–cell adhesion in the near wall region under dynamic shear forces has been studied. In particular, we focus on neutrophil (PMN)–melanoma cell emboli formation in a non-linear shear flow and subsequent tethering to the vascular endothelium (EC) as a result of cell–cell aggregation. The extent of tumor cell adhesion to a vessel wall is governed by the kinetic formation/disruption of receptor–ligand bonds, soluble signaling proteins within the tumor microenvironment, and the hydrodynamic shear within the circulation. Upon tumor cell arrest on the endothelium, retraction of EC during tumor cell extravasation occurs due to the disruption of intercellular channels or disassembly of the vascular endothelial (VE)-cadherin homodimers that allow the passage of soluble proteins and cells. Preliminary studies have found tumor-elicited PMNs increase melanoma cell extravasation, which involves PMNs tethering on the EC and subsequently capturing/maintaining melanoma cells in close proximity to the EC. Results have indicated a novel finding that PMN-facilitated melanoma cell arrest on the EC is mediated by binding between the intercellular adhesion molecule (ICAM)-1 (expressing on both melanoma cells and ECs) and β2 integrins on PMNs, influenced by tumor-induced inflammatory cytokines, e.g., interleukin (IL)-8, and hydrodynamic shear rates. Furthermore, the adherens junctions in terms of VE-cadherin are regulated by endothelial mitogen activated protein kinases (MAPK) in response to tumor cell adhesion to the EC, as well as to IL-8 and several other soluble signaling proteins within the tumor microenvironment. These studies will yield new evidence for the complex role of hemodynamics, protein signaling, and heterotypic cell adhesion in the recruitment of metastatic cancer cells to the EC in the microcirculation during metastasis, which will be significant in fostering new cross-disciplinary approaches to cancer treatment.
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U2 - 10.1007/8415_2010_21
DO - 10.1007/8415_2010_21
M3 - Chapter
AN - SCOPUS:85085181517
T3 - Studies in Mechanobiology, Tissue Engineering and Biomaterials
SP - 477
EP - 521
BT - Studies in Mechanobiology, Tissue Engineering and Biomaterials
PB - Springer
ER -