Administration of recombinant human insulin-like growth factor I to pigs: Determination of circulating half-lives and chomatographic profiles

P. E. Walton, R. Gopinath, B. D. Burleigh, Terry D. Etherton

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Abstract

Recombinant human insulin-like growth factor I (IGF-I) was injected daily (4 or 8 mg/pig) as an intra-arterial bolus into pigs for 3 consecutive days and the serum IGF-I concentration was measured to determine disappearance profiles. IGF-I partitioned to a fast component (half-life, t( 1/2 ) = 5.7 min) and a slow component (t( 1/2 ) = 253 min) in pig serum. Chromatography of serum revealed that the fast component comprised unbound IGF-I, whereas the slow component comprised IGF-I bound to 40- and 150-kD serum IGF-binding proteins. In addition, administration of exogenous IGF-I caused significant hypoglycemia.

Original languageEnglish (US)
Pages (from-to)138-142
Number of pages5
JournalHormone Research
Volume31
Issue number3
DOIs
Publication statusPublished - Jan 1 1989

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All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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