Adoptive transfer of induced-treg cells effectively attenuates murine airway allergic inflammation

Wei Xu, Qin Lan, Maogen Chen, Hui Chen, Ning Zhu, Xiaohui Zhou, Julie Wang, Huimin Fan, Chun Song Yan, Jiu Long Kuang, David Warburton, Dieudonnée Togbe, Bernhard Ryffel, Song Guo Zheng, Wei Shi

Research output: Contribution to journalArticle

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Abstract

Both nature and induced regulatory T (Treg) lymphocytes are potent regulators of autoimmune and allergic disorders. Defects in endogenous Treg cells have been reported in patients with allergic asthma, suggesting that disrupted Treg cell-mediated immunological regulation may play an important role in airway allergic inflammation. In order to determine whether adoptive transfer of induced Treg cells generated in vitro can be used as an effective therapeutic approach to suppress airway allergic inflammation, exogenously induced Treg cells were infused into ovalbumin-sensitized mice prior to or during intranasal ovalbumin challenge. The results showed that adoptive transfer of induced Treg cells prior to allergen challenge markedly reduced airway hyperresponsiveness, eosinophil recruitment, mucus hyper-production, airway remodeling, and IgE levels. This effect was associated with increase of Treg cells (CD4+FoxP3+) and decrease of dendritic cells in the draining lymph nodes, and with reduction of Th1, Th2, and Th17 cell response as compared to the controls. Moreover, adoptive transfer of induced Treg cells during allergen challenge also effectively attenuate airway inflammation and improve airway function, which are comparable to those by natural Treg cell infusion. Therefore, adoptive transfer of in vitro induced Treg cells may be a promising therapeutic approach to prevent and treat severe asthma.

Original languageEnglish (US)
Article numbere40314
JournalPloS one
Volume7
Issue number7
DOIs
StatePublished - Jul 9 2012

Fingerprint

Adoptive Transfer
Regulatory T-Lymphocytes
inflammation
Inflammation
mice
Ovalbumin
cells
Allergens
ovalbumin
asthma
allergens
Lymphocytes
Asthma
Immunoglobulin E
Airway Remodeling
Th17 Cells
therapeutics
Th2 Cells
Th1 Cells
Mucus

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Xu, Wei ; Lan, Qin ; Chen, Maogen ; Chen, Hui ; Zhu, Ning ; Zhou, Xiaohui ; Wang, Julie ; Fan, Huimin ; Yan, Chun Song ; Kuang, Jiu Long ; Warburton, David ; Togbe, Dieudonnée ; Ryffel, Bernhard ; Zheng, Song Guo ; Shi, Wei. / Adoptive transfer of induced-treg cells effectively attenuates murine airway allergic inflammation. In: PloS one. 2012 ; Vol. 7, No. 7.
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abstract = "Both nature and induced regulatory T (Treg) lymphocytes are potent regulators of autoimmune and allergic disorders. Defects in endogenous Treg cells have been reported in patients with allergic asthma, suggesting that disrupted Treg cell-mediated immunological regulation may play an important role in airway allergic inflammation. In order to determine whether adoptive transfer of induced Treg cells generated in vitro can be used as an effective therapeutic approach to suppress airway allergic inflammation, exogenously induced Treg cells were infused into ovalbumin-sensitized mice prior to or during intranasal ovalbumin challenge. The results showed that adoptive transfer of induced Treg cells prior to allergen challenge markedly reduced airway hyperresponsiveness, eosinophil recruitment, mucus hyper-production, airway remodeling, and IgE levels. This effect was associated with increase of Treg cells (CD4+FoxP3+) and decrease of dendritic cells in the draining lymph nodes, and with reduction of Th1, Th2, and Th17 cell response as compared to the controls. Moreover, adoptive transfer of induced Treg cells during allergen challenge also effectively attenuate airway inflammation and improve airway function, which are comparable to those by natural Treg cell infusion. Therefore, adoptive transfer of in vitro induced Treg cells may be a promising therapeutic approach to prevent and treat severe asthma.",
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Xu, W, Lan, Q, Chen, M, Chen, H, Zhu, N, Zhou, X, Wang, J, Fan, H, Yan, CS, Kuang, JL, Warburton, D, Togbe, D, Ryffel, B, Zheng, SG & Shi, W 2012, 'Adoptive transfer of induced-treg cells effectively attenuates murine airway allergic inflammation', PloS one, vol. 7, no. 7, e40314. https://doi.org/10.1371/journal.pone.0040314

Adoptive transfer of induced-treg cells effectively attenuates murine airway allergic inflammation. / Xu, Wei; Lan, Qin; Chen, Maogen; Chen, Hui; Zhu, Ning; Zhou, Xiaohui; Wang, Julie; Fan, Huimin; Yan, Chun Song; Kuang, Jiu Long; Warburton, David; Togbe, Dieudonnée; Ryffel, Bernhard; Zheng, Song Guo; Shi, Wei.

In: PloS one, Vol. 7, No. 7, e40314, 09.07.2012.

Research output: Contribution to journalArticle

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AU - Xu, Wei

AU - Lan, Qin

AU - Chen, Maogen

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AU - Zhou, Xiaohui

AU - Wang, Julie

AU - Fan, Huimin

AU - Yan, Chun Song

AU - Kuang, Jiu Long

AU - Warburton, David

AU - Togbe, Dieudonnée

AU - Ryffel, Bernhard

AU - Zheng, Song Guo

AU - Shi, Wei

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N2 - Both nature and induced regulatory T (Treg) lymphocytes are potent regulators of autoimmune and allergic disorders. Defects in endogenous Treg cells have been reported in patients with allergic asthma, suggesting that disrupted Treg cell-mediated immunological regulation may play an important role in airway allergic inflammation. In order to determine whether adoptive transfer of induced Treg cells generated in vitro can be used as an effective therapeutic approach to suppress airway allergic inflammation, exogenously induced Treg cells were infused into ovalbumin-sensitized mice prior to or during intranasal ovalbumin challenge. The results showed that adoptive transfer of induced Treg cells prior to allergen challenge markedly reduced airway hyperresponsiveness, eosinophil recruitment, mucus hyper-production, airway remodeling, and IgE levels. This effect was associated with increase of Treg cells (CD4+FoxP3+) and decrease of dendritic cells in the draining lymph nodes, and with reduction of Th1, Th2, and Th17 cell response as compared to the controls. Moreover, adoptive transfer of induced Treg cells during allergen challenge also effectively attenuate airway inflammation and improve airway function, which are comparable to those by natural Treg cell infusion. Therefore, adoptive transfer of in vitro induced Treg cells may be a promising therapeutic approach to prevent and treat severe asthma.

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