Advances in de novo strain design using integrated systems and synthetic biology tools

Chiam Yu Ng, Ali Khodayari, Anupam Chowdhury, Costas D. Maranas

Research output: Contribution to journalReview article

17 Scopus citations

Abstract

Recent efforts in expanding the range of biofuel and biorenewable molecules using microbial production hosts have focused on the introduction of non-native pathways in model organisms and the bio-prospecting of non-model organisms with desirable features. Current challenges lie in the assembly and coordinated expression of the (non-)native pathways and the elimination of competing pathways and undesirable regulation. Several systems and synthetic biology approaches providing contrasting top-down and bottom-up strategies, respectively, have been developed. In this review, we discuss recent advances in both in silico and experimental approaches for metabolic pathway design and engineering, with a critical assessment of their merits and remaining challenges.

Original languageEnglish (US)
Pages (from-to)105-114
Number of pages10
JournalCurrent Opinion in Chemical Biology
Volume28
DOIs
StatePublished - Oct 1 2015

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

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