Advances in transformation technology for vegetable brassica

E. D. Earle, T. D. Metz, R. T. Roush, A. M. Shelton

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Although transformation of Brassica vegetables has lagged behind similar work with rapeseed, transgenic cauliflower, broccoli, cabbage, and kale plants have been obtained by several methods. The most general approach is use of Agrobcicterium tumefaciens. A U.S. patent for A. tumefaciens-mediatcd transformation of Brassica; including B. oleracea, was recently granted to Calgene, Inc. Transformation via A. rhizogenes is another option, but the plants recovered may show abnormal phenotypes. Direct DNA update into protoplasts, induced either by polyethylene glycol or electroporation, has also succeeded. Biolistic approaches have not yet played an important role in vegetable Brassica transformation. Even the successful procedures are still not routine, and transgenic plants are usually recovered from fewer than 10% of explants transformed. Control of ethylene and moisture levels in culture plates are among the factors that can increase efficiency. Transformants are most often selected by resistance to kanamycin or hygromycin. Other genes introduced include B-glucuronidase, genes for resistance to herbicides, an S-locus gene, and insecticidal protein genes from Bacillus thuringiensis (Bt). When a modified CrylA(c) Bt gene was introduced into broccoli and cabbage, about 70% of ca. 250 transformants recovered were resistant to diamondback moths, a major pest of crucifers. Progeny of some of these transgenic plants are now being used in tests of insect resistance management strategies involving refuges. Additional transgenic vegetable Brassicas with enhanced resistances or other horticultural improvements are likely to be available soon, but regulatory issues will delay their commercial release.

Original languageEnglish (US)
Pages (from-to)161-168
Number of pages8
JournalActa Horticulturae
Volume407
Issue number1
StatePublished - Dec 1 1996

Fingerprint

Brassica
vegetables
broccoli
genes
Bacillus thuringiensis
cabbage
transgenic plants
genetically modified organisms
biolistics
insecticidal proteins
kale
electroporation
resistance management
herbicide resistance
Plutella xylostella
cauliflower
patents
kanamycin
Brassica oleracea
rapeseed

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

Earle, E. D., Metz, T. D., Roush, R. T., & Shelton, A. M. (1996). Advances in transformation technology for vegetable brassica. Acta Horticulturae, 407(1), 161-168.
Earle, E. D. ; Metz, T. D. ; Roush, R. T. ; Shelton, A. M. / Advances in transformation technology for vegetable brassica. In: Acta Horticulturae. 1996 ; Vol. 407, No. 1. pp. 161-168.
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Earle, ED, Metz, TD, Roush, RT & Shelton, AM 1996, 'Advances in transformation technology for vegetable brassica', Acta Horticulturae, vol. 407, no. 1, pp. 161-168.

Advances in transformation technology for vegetable brassica. / Earle, E. D.; Metz, T. D.; Roush, R. T.; Shelton, A. M.

In: Acta Horticulturae, Vol. 407, No. 1, 01.12.1996, p. 161-168.

Research output: Contribution to journalArticle

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Earle ED, Metz TD, Roush RT, Shelton AM. Advances in transformation technology for vegetable brassica. Acta Horticulturae. 1996 Dec 1;407(1):161-168.