Aerobic degradation of tetrachloroethylene by toluene-o-xylene monooxygenase of Pseudomonas stutzeri OX1

Doohyun Ryoo, Hojae Shim, Keith Canada, Paola Barbieri, Thomas K. Wood

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Tetrachloroethylene (PCE) is thought to have no natural source, so it is one of the most difficult contaminants to degrade biologically. This common groundwater pollutant was thought completely nonbiodegradable in the presence of oxygen. Here we report that the wastewater bacterium Pseudomonas stutzeri OX1 degrades aerobically 0.56 μmol of 2.0 μmol PCE in 21 h (V(max) ≃ 2.5 nmol min-1 mg-1 protein and K(M) ≃ 34 μM). These results were corroborated by the generation of 0.48 μmol of the degradation product, chloride ions. This degradation was confirmed to be a result of expression of toluene-o-xylene monooxygenase (ToMO) by P. stutzeti OX1, since cloning and expressing this enzyme in Escherichia coli led to the aerobic degradation of 0.19 μmol of 2.0 μmol PCE and the generation of stoichiometric amounts of chloride. In addition, PCE induces formation of ToMO, which leads to its own degradation in P. stutzeri OX1. Degradation intermediates reduce the growth rate of this strain by 27%.

Original languageEnglish (US)
Pages (from-to)775-778
Number of pages4
JournalNature Biotechnology
Volume18
Issue number7
DOIs
StatePublished - Jul 1 2000

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Pseudomonas stutzeri
Tetrachloroethylene
Xylene
Toluene
Chlorides
Degradation
Groundwater
Waste Water
Organism Cloning
Ions
Oxygen
Escherichia coli
Bacteria
Enzymes
Growth
Cloning
Wastewater
toluene 2-xylene monooxygenase
Impurities
Proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

Cite this

Ryoo, Doohyun ; Shim, Hojae ; Canada, Keith ; Barbieri, Paola ; Wood, Thomas K. / Aerobic degradation of tetrachloroethylene by toluene-o-xylene monooxygenase of Pseudomonas stutzeri OX1. In: Nature Biotechnology. 2000 ; Vol. 18, No. 7. pp. 775-778.
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Aerobic degradation of tetrachloroethylene by toluene-o-xylene monooxygenase of Pseudomonas stutzeri OX1. / Ryoo, Doohyun; Shim, Hojae; Canada, Keith; Barbieri, Paola; Wood, Thomas K.

In: Nature Biotechnology, Vol. 18, No. 7, 01.07.2000, p. 775-778.

Research output: Contribution to journalArticle

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