Affinity grid-based cryo-EM of PKC binding to RACK1 on the ribosome

Gyanesh Sharma, Jesper Pallesen, Sanchaita Das, Robert Grassucci, Robert Langlois, Cheri M. Hampton, Deborah F. Kelly, Amedee des Georges, Joachim Frank

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Abstract

Affinity grids (AG) are specialized EM grids that bind macromolecular complexes containing tagged proteins to obtain maximum occupancy for structural analysis through single-particle EM. In this study, utilizing AG, we show that His-tagged activated PKC βII binds to the small ribosomal subunit (40S). We reconstructed a cryo-EM map which shows that PKC βII interacts with RACK1, a seven-bladed β-propeller protein present on the 40S and binds in two different regions close to blades 3 and 4 of RACK1. This study is a first step in understanding the molecular framework of PKC βII/RACK1 interaction and its role in translation.

Original languageEnglish (US)
Pages (from-to)190-194
Number of pages5
JournalJournal of Structural Biology
Volume181
Issue number2
DOIs
StatePublished - Feb 2013

All Science Journal Classification (ASJC) codes

  • Structural Biology

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    Sharma, G., Pallesen, J., Das, S., Grassucci, R., Langlois, R., Hampton, C. M., Kelly, D. F., des Georges, A., & Frank, J. (2013). Affinity grid-based cryo-EM of PKC binding to RACK1 on the ribosome. Journal of Structural Biology, 181(2), 190-194. https://doi.org/10.1016/j.jsb.2012.11.006