Affinity-purified escherichia coli j5 lipopolysaccharide-specific igg protects neutropenic rats against gram-negative bacterial sepsis

Apurba K. Bhattacharjee, Steven M. Opal, John E. Palardy, Joseph J. Drabick, Hugh Collins, Robert Taylor, Amos Cotton, Alan S. Cross

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Antibodies were raised in rabbits by immunization with the heat-killed J5 mutant ofEscherichia coli 0111 (Rc chemotype). Serum antibodies were separated into purified IgG and IgM by sequential affinity chromatography on protein G-Sepharose and anti-rabbit IgG-Sepharose columns. J5 lipopolysaccharide (LPS)-specific IgG was prepared by affinity chromatography of purified IgG on a J5 LPS-EAH Sepharose 4B affinity column. Purified IgM, IgG, and J5 LPS-specific IgG protected neutropenic rats against lethal challenge with Pseudomonas aeruginosa 12:4:4 (Fisher Devlin immunotype 6). Nine of 16 rats treated with the IgM fraction were protected (P <.001). Thirteen of 20 rats treated with the purified IgG and 6 of 8 treated with J5 LPS-specific IgG were protected compared with none of 25 treated with IgG made from the preimmune serum of the same rabbit (P <.001). These results demonstrate that purified J5 LPS-specific IgG protects against the lethal consequences of gram-negative bacteremia.

Original languageEnglish (US)
Pages (from-to)622-629
Number of pages8
JournalJournal of Infectious Diseases
Volume170
Issue number3
DOIs
StatePublished - Sep 1994

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Lipopolysaccharides
Sepsis
Immunoglobulin G
Escherichia coli
Immunoglobulin M
Rabbits
Affinity Chromatography
Sepharose
Antibodies
Bacteremia
Serum
Pseudomonas aeruginosa
Immunization
Hot Temperature
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Bhattacharjee, Apurba K. ; Opal, Steven M. ; Palardy, John E. ; Drabick, Joseph J. ; Collins, Hugh ; Taylor, Robert ; Cotton, Amos ; Cross, Alan S. / Affinity-purified escherichia coli j5 lipopolysaccharide-specific igg protects neutropenic rats against gram-negative bacterial sepsis. In: Journal of Infectious Diseases. 1994 ; Vol. 170, No. 3. pp. 622-629.
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Affinity-purified escherichia coli j5 lipopolysaccharide-specific igg protects neutropenic rats against gram-negative bacterial sepsis. / Bhattacharjee, Apurba K.; Opal, Steven M.; Palardy, John E.; Drabick, Joseph J.; Collins, Hugh; Taylor, Robert; Cotton, Amos; Cross, Alan S.

In: Journal of Infectious Diseases, Vol. 170, No. 3, 09.1994, p. 622-629.

Research output: Contribution to journalArticle

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AU - Bhattacharjee, Apurba K.

AU - Opal, Steven M.

AU - Palardy, John E.

AU - Drabick, Joseph J.

AU - Collins, Hugh

AU - Taylor, Robert

AU - Cotton, Amos

AU - Cross, Alan S.

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N2 - Antibodies were raised in rabbits by immunization with the heat-killed J5 mutant ofEscherichia coli 0111 (Rc chemotype). Serum antibodies were separated into purified IgG and IgM by sequential affinity chromatography on protein G-Sepharose and anti-rabbit IgG-Sepharose columns. J5 lipopolysaccharide (LPS)-specific IgG was prepared by affinity chromatography of purified IgG on a J5 LPS-EAH Sepharose 4B affinity column. Purified IgM, IgG, and J5 LPS-specific IgG protected neutropenic rats against lethal challenge with Pseudomonas aeruginosa 12:4:4 (Fisher Devlin immunotype 6). Nine of 16 rats treated with the IgM fraction were protected (P <.001). Thirteen of 20 rats treated with the purified IgG and 6 of 8 treated with J5 LPS-specific IgG were protected compared with none of 25 treated with IgG made from the preimmune serum of the same rabbit (P <.001). These results demonstrate that purified J5 LPS-specific IgG protects against the lethal consequences of gram-negative bacteremia.

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