Age-associated distribution of antimicrobial-resistant salmonella enterica and escherichia coli isolated from dairy herds in pennsylvania, 2013-2015

Huilin Cao, Abani K. Pradhan, Jeffrey S. Karns, Ernest Peter Hovingh, David R. Wolfgang, Bryan T. Vinyard, Seon Woo Kim, Serajus Salaheen, Bradd J. Haley, Jo Ann S. Van Kessel

Research output: Contribution to journalReview article

3 Citations (Scopus)

Abstract

Antimicrobial resistance has become a major global public health concern, and agricultural operations are often implicated as a source of resistant bacteria. This study characterized the prevalence of antimicrobial-resistant Salmonella enterica and Escherichia coli from a total of 443 manure composite samples from preweaned calves, postweaned calves, dry cows, and lactating cows from 80 dairy operations in Pennsylvania. A total of 1095 S. enterica and 2370 E. coli isolates were screened and tested for resistance to 14 antimicrobials on the National Antimicrobial Resistance Monitoring System Gram-negative (NARMS GN) panel. Salmonellae were isolated from 67% of dairy operations, and 99% of the isolates were pan-susceptible. Salmonella were isolated more frequently from lactating and dry cow samples than from pre- and postweaned calf samples. Overall, the most prevalent serotypes were Cerro, Montevideo, Kentucky, and Newport. E. coli were isolated from all the manure composite samples, and isolates were commonly resistant to tetracyclines, sulfonamides, and aminoglycosides. Resistance was detected more frequently in the E. coli isolates from pre- and postweaned calf samples than in isolates from dry and lactating cow samples (p < 0.05). Multidrug-resistant E. coli (i.e., resistant to >3 antimicrobial classes) were isolated from 66 farms (83%) with significantly greater prevalence in preweaned calves (p < 0.05) than in the older age groups. The bla CTX-M and bla CMY genes were detected in the cephalosporin-resistant E. coli from 4% and 35% of the farms, respectively. These findings indicate that dairy animals, especially the calf population, serve as significant reservoirs for antimicrobial-resistant bacteria. Additional research on the colonization and persistence of resistant E. coli in calves is warranted to identify potential avenues for mitigation.

Original languageEnglish (US)
Pages (from-to)60-67
Number of pages8
JournalFoodborne pathogens and disease
Volume16
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

Salmonella enterica
Age Distribution
dairy herds
anti-infective agents
calves
Escherichia coli
Manure
cows
Salmonella
sampling
antibiotic resistance
dairies
Bacteria
Tetracyclines
dairy animals
Sulfonamides
tetracyclines
Aminoglycosides
aminoglycosides
cephalosporins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Animal Science and Zoology

Cite this

Cao, Huilin ; Pradhan, Abani K. ; Karns, Jeffrey S. ; Hovingh, Ernest Peter ; Wolfgang, David R. ; Vinyard, Bryan T. ; Kim, Seon Woo ; Salaheen, Serajus ; Haley, Bradd J. ; Van Kessel, Jo Ann S. / Age-associated distribution of antimicrobial-resistant salmonella enterica and escherichia coli isolated from dairy herds in pennsylvania, 2013-2015. In: Foodborne pathogens and disease. 2019 ; Vol. 16, No. 1. pp. 60-67.
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title = "Age-associated distribution of antimicrobial-resistant salmonella enterica and escherichia coli isolated from dairy herds in pennsylvania, 2013-2015",
abstract = "Antimicrobial resistance has become a major global public health concern, and agricultural operations are often implicated as a source of resistant bacteria. This study characterized the prevalence of antimicrobial-resistant Salmonella enterica and Escherichia coli from a total of 443 manure composite samples from preweaned calves, postweaned calves, dry cows, and lactating cows from 80 dairy operations in Pennsylvania. A total of 1095 S. enterica and 2370 E. coli isolates were screened and tested for resistance to 14 antimicrobials on the National Antimicrobial Resistance Monitoring System Gram-negative (NARMS GN) panel. Salmonellae were isolated from 67{\%} of dairy operations, and 99{\%} of the isolates were pan-susceptible. Salmonella were isolated more frequently from lactating and dry cow samples than from pre- and postweaned calf samples. Overall, the most prevalent serotypes were Cerro, Montevideo, Kentucky, and Newport. E. coli were isolated from all the manure composite samples, and isolates were commonly resistant to tetracyclines, sulfonamides, and aminoglycosides. Resistance was detected more frequently in the E. coli isolates from pre- and postweaned calf samples than in isolates from dry and lactating cow samples (p < 0.05). Multidrug-resistant E. coli (i.e., resistant to >3 antimicrobial classes) were isolated from 66 farms (83{\%}) with significantly greater prevalence in preweaned calves (p < 0.05) than in the older age groups. The bla CTX-M and bla CMY genes were detected in the cephalosporin-resistant E. coli from 4{\%} and 35{\%} of the farms, respectively. These findings indicate that dairy animals, especially the calf population, serve as significant reservoirs for antimicrobial-resistant bacteria. Additional research on the colonization and persistence of resistant E. coli in calves is warranted to identify potential avenues for mitigation.",
author = "Huilin Cao and Pradhan, {Abani K.} and Karns, {Jeffrey S.} and Hovingh, {Ernest Peter} and Wolfgang, {David R.} and Vinyard, {Bryan T.} and Kim, {Seon Woo} and Serajus Salaheen and Haley, {Bradd J.} and {Van Kessel}, {Jo Ann S.}",
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Age-associated distribution of antimicrobial-resistant salmonella enterica and escherichia coli isolated from dairy herds in pennsylvania, 2013-2015. / Cao, Huilin; Pradhan, Abani K.; Karns, Jeffrey S.; Hovingh, Ernest Peter; Wolfgang, David R.; Vinyard, Bryan T.; Kim, Seon Woo; Salaheen, Serajus; Haley, Bradd J.; Van Kessel, Jo Ann S.

In: Foodborne pathogens and disease, Vol. 16, No. 1, 01.01.2019, p. 60-67.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Age-associated distribution of antimicrobial-resistant salmonella enterica and escherichia coli isolated from dairy herds in pennsylvania, 2013-2015

AU - Cao, Huilin

AU - Pradhan, Abani K.

AU - Karns, Jeffrey S.

AU - Hovingh, Ernest Peter

AU - Wolfgang, David R.

AU - Vinyard, Bryan T.

AU - Kim, Seon Woo

AU - Salaheen, Serajus

AU - Haley, Bradd J.

AU - Van Kessel, Jo Ann S.

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N2 - Antimicrobial resistance has become a major global public health concern, and agricultural operations are often implicated as a source of resistant bacteria. This study characterized the prevalence of antimicrobial-resistant Salmonella enterica and Escherichia coli from a total of 443 manure composite samples from preweaned calves, postweaned calves, dry cows, and lactating cows from 80 dairy operations in Pennsylvania. A total of 1095 S. enterica and 2370 E. coli isolates were screened and tested for resistance to 14 antimicrobials on the National Antimicrobial Resistance Monitoring System Gram-negative (NARMS GN) panel. Salmonellae were isolated from 67% of dairy operations, and 99% of the isolates were pan-susceptible. Salmonella were isolated more frequently from lactating and dry cow samples than from pre- and postweaned calf samples. Overall, the most prevalent serotypes were Cerro, Montevideo, Kentucky, and Newport. E. coli were isolated from all the manure composite samples, and isolates were commonly resistant to tetracyclines, sulfonamides, and aminoglycosides. Resistance was detected more frequently in the E. coli isolates from pre- and postweaned calf samples than in isolates from dry and lactating cow samples (p < 0.05). Multidrug-resistant E. coli (i.e., resistant to >3 antimicrobial classes) were isolated from 66 farms (83%) with significantly greater prevalence in preweaned calves (p < 0.05) than in the older age groups. The bla CTX-M and bla CMY genes were detected in the cephalosporin-resistant E. coli from 4% and 35% of the farms, respectively. These findings indicate that dairy animals, especially the calf population, serve as significant reservoirs for antimicrobial-resistant bacteria. Additional research on the colonization and persistence of resistant E. coli in calves is warranted to identify potential avenues for mitigation.

AB - Antimicrobial resistance has become a major global public health concern, and agricultural operations are often implicated as a source of resistant bacteria. This study characterized the prevalence of antimicrobial-resistant Salmonella enterica and Escherichia coli from a total of 443 manure composite samples from preweaned calves, postweaned calves, dry cows, and lactating cows from 80 dairy operations in Pennsylvania. A total of 1095 S. enterica and 2370 E. coli isolates were screened and tested for resistance to 14 antimicrobials on the National Antimicrobial Resistance Monitoring System Gram-negative (NARMS GN) panel. Salmonellae were isolated from 67% of dairy operations, and 99% of the isolates were pan-susceptible. Salmonella were isolated more frequently from lactating and dry cow samples than from pre- and postweaned calf samples. Overall, the most prevalent serotypes were Cerro, Montevideo, Kentucky, and Newport. E. coli were isolated from all the manure composite samples, and isolates were commonly resistant to tetracyclines, sulfonamides, and aminoglycosides. Resistance was detected more frequently in the E. coli isolates from pre- and postweaned calf samples than in isolates from dry and lactating cow samples (p < 0.05). Multidrug-resistant E. coli (i.e., resistant to >3 antimicrobial classes) were isolated from 66 farms (83%) with significantly greater prevalence in preweaned calves (p < 0.05) than in the older age groups. The bla CTX-M and bla CMY genes were detected in the cephalosporin-resistant E. coli from 4% and 35% of the farms, respectively. These findings indicate that dairy animals, especially the calf population, serve as significant reservoirs for antimicrobial-resistant bacteria. Additional research on the colonization and persistence of resistant E. coli in calves is warranted to identify potential avenues for mitigation.

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